Project/Area Number |
08556012
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 展開研究 |
Research Field |
応用微生物学・応用生物化学
|
Research Institution | The University of Tokyo |
Principal Investigator |
OHTA Akinori The University of Tokyo, Graduate School of Agriculture & Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (30125885)
|
Co-Investigator(Kenkyū-buntansha) |
ISHIDA Keiko NEDO,Alcohol Production Head Office, Research & Development Center, Investigator, アルコール事業本部・研究開発センター, 主任
TAKAGI Yoshio NEDO,Alcohol Production Head Office, Research & Development Center, Manager, アルコール事業本部・研究開発センター, 課長
SAIKI Takashi New Energy & Industrial Technology Development Organization (NEDO), Alcohol Prod, アルコール事業本部・研究開発センター, 所長
|
Project Period (FY) |
1996 – 1997
|
Project Status |
Completed (Fiscal Year 1997)
|
Budget Amount *help |
¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥3,700,000 (Direct Cost: ¥3,700,000)
|
Keywords | Saccharomyces cerevisiae / yeast / alcohol fermentation / self cloning / flocculation / FL01 / yeast breeding / targeted gene manipulation / セルフクローニング / Saccharomyos cerevisiae / 凝集性 / アルコール生産 / 酵母 |
Research Abstract |
The control of yeast aggregation is desirable for alcohol fermentation industry, because flocculent yeast by aggregation can be easily removed from the fermenter and reused for next successive fermentation. Aiming development of such a technique, we introduced FL01, a Saccharomyces cerevisiae gene that causes flocculation, into an industrial alcohol-fermenting S.Cerevisiae strain 396. In consideration with inevitable escape of yeast cells form large scale of fermentation and the purpose of the pruduct alcohol for food and pharmaceuticals, it is desirable that the fermenting yeast does not contain heterologous genes. We, therefore, inserted the homologous ADH1^P-FL01 gene into URA3 of the strain 396. The resultant recombinat was flocculent and comparable in capability of alcohol production with the parental industrial S.cerevisiae strain 396.
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