| Project/Area Number |
08670986
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | JIKEI UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
NOMURA Nakao JIKEI UNIVERSITY SCHOOL OF MEDICINE,The medical department, assistant, 医学部, 助手 (50198628)
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| Co-Investigator(Kenkyū-buntansha) |
FUSE Nobuko JIKEI UNIVERSITY SCHOOL OF MEDICINE,The medical department, assistant, 医学部, 助手 (60277077)
SUZUKI Arata JIKEI UNIVERSITY SCHOOL OF MEDICINE,The medical department, assistant, 医学部, 助手 (50277042)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Ferrochelatase / Eythropietic protoporphyria |
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| Research Abstract |
Lymphocytes of erythropoietic protoporphyria(EPP) patients were infected with EB virus and lymphoblastoid cell lines were established. In each case, porphyrin levels in erythrocytes and ferrochelatase activities were assayed by spectrofluorometer and HPLC.These Data are acumulating in the database to analyze the relationship between the clinical histories such as onsets, degrees of photosensitivies and the laboratory data statistically.
cDNA was made by RT-PCR using mRNA which was extracted from lymphoblastoid cell lines and its sequence analysis is on going. But we do not have enough outcome or reach meaningful conclusions unfortunately. Allele specific oligomer assay, relationship between each mutation and clinical severity or ferrochelatase activity which was expressed in Chinese overy cells are planed. Furthermore, [2F-2S] cluster in three dimensional structure will be analyzed in normal and mutant DNA made by site-directed mutagenesis.
We had additional projects in this term. Articles about the serum porphyrin levels in the patients with HIV and/or HCV the effect of soluble complement receptor type 1 on porphyria models and the mutation analysis of hereditary coproporphyria were published.
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