Targetting chemotherapy of brain tumor using liposome encapsulated cisplatin and novel cisplatin derivative
| Project/Area Number |
08671591
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
SHIBATA Shobu Nagasaki University, School of Medicine, professor, 医学部, 教授 (50039517)
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| Co-Investigator(Kenkyū-buntansha) |
RYU Tobutoshi Nagasaki University, School of Medicine, Assistant, 医学部, 助手 (20231706)
TOKUNAGA Yoshiharu Nagasaki University, School of Medicine Hospital, Lecturer, 医学部・附属病院, 講師 (00207557)
YASUNAGA Akio Nagasaki University, School of Medicine, Associated Professor, 医学部, 助教授 (90124841)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Brain tumor / Lipsome encapulated cisplatin / Cisplatin derivative |
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| Research Abstract |
Antitumor effect of polysaccharide-coated liposome, 1-aminolactose pullulan-coated liposome encapsulated cisplatin and a newly synthesized cisplatin derivative, 4-pyridoxate diammine hydroxy platinum (Pypt), against brain tumours in vitro and in vivo were evaluated and compared with cisplatin.
(1)When pullulan-coated liposome labeled with ^<14>C was intracarotidly administrated into the 9L cells in planted brain tumour rats, brain tumours uptake of radioactivity increased 4.5 times and spleen uptake decrease compared with the neutral liposome. The cytotoxity of cisplatin against 9L-glioma cell was not inhibited by encapsulation of pullulan-coated liposomes. The median survival times for 9L glioma rat model following intracarotid administration of pullulan-coated liposome encapsulated cisplatin were 35.5 days and 20.3 days for control (P<0.05). The quantity of platinum of 1-aminolactose pullulan-coated liposome encapsulated cisplatin into the brain tumour was about 4 times as great as that of pullulan-coated liposome encapsulated cisplatin.
(2)Rat bran was inoculated with 9L glioma, and Pypt and cisplatin were administered as selective intracarotid infusions. A significant improvement in survival time compared with control animals was found. Platinum concentration in the brain tumours, 30 min after administration of Pypt, was 2.4 times significantly higher than with cisplatin. This suggest that drug delivery efficiency to the 9L glioma is greater for Pypt than cisplatin.
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Report
(3 results)
Research Products
(11 results)
