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A study for the suppressive effect of the peptide growth factors on radiation-induced apoptosis.

Research Project

Project/Area Number 08680576
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

KATOH Osamu  Research Institute for Radiation Biology and Medicine HIROSHIMA UNIVERSITY Associate professor, 原爆放射能医学研究所, 助教授 (90214361)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsVEGF / apoptosis / bel family / transcription factor / zinc-finger motif
Research Abstract

We have found that peptide growth factors such as VEGF suppress the apoptotic cell death of the hematopoietic cells induced by exposure to gamma-rays.
1. To elucidate the molecular mechanisns of the suppressive effects of VEGF,we examined the mRNA level and the protein level of the bel family (BCL-2, BCL-XL,MCL-1) by Northern blot and Western blot hybridization analysis.
The mRNA level and the protein level of MCL-1 were increased in the CMK86 cells, a human leukemia cell line, stimulated by VEGF.Those of BCL-2 or BCL-XL,however, did not change by VEGF.MCL-1 may be involved in the suppressive effect of VEGF on apoptotic cell death.
2. We cloned a novel Kruppel-type zinc finger gene ZK-7, induced by VEGF from a CMK86 cDNA library. The deduced ZK-7 protein has 298 amino acids, and has KRAB (Kruppel associated box) -A box at the N-terminus and seven C_2H_2-type zinc-finger domain at the C-terminus. The function of the ZK-7 gene on the signaling pathway of popsis remains to be elucidated.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] ROZENSON, R.I.: "セミパラチンクス住民における電離放射線被曝の発癌におよぼす影響" 広島医学. 49. 299-302 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 加藤 修: "γ線誘導アポトーシスに対して修飾効果を有する細胞増殖因子の検討" 広島医学. 49. 441-443 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] KAWAISHI, K.: "Decreased L-selectin expression in CD34-Positive cells from patients with chronic myelocytic leukemia" Br. J. Haematol. 93. 367-374 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] OHASHI, N.: "No alterlation in DNA topoisomerase 1 gene related to CPT-11 resistance in human lung cancer" Jap. J. Cancer Res. 87. 1280-1287 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 加藤 修: "細胞増殖因子のガンマ線誘導アポトーシス抑制機構について" 長崎医学会雑誌. 71. 329-331 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] NAKAGAWA, Y.: "Colon tissue implanted into the glandular stomach in rats lack susceptibility to N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) carcinogenesis" Oncology Reports. 4. 517-519 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] WATANABE, H.: "Induction of insulinomas and kidney tumors in SD male rats by x-irradiation of gastric region" J Toxicol Pathol. 10. 77-81 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] KIMURA, A.: "c-kit point mutation in patients with myeloproliferative disorders" Leukemia and Lymphoma. 25. 281-287 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] MORITA, E.: "A novel point mutation in the steroid sulfatase gene in x-linked ichthyosis" J Invest Dermatol. 109. 244-245 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] TAKAHASHI, T.: "The role of glucuronidation in 7-Ethyl-10-hydroxycamptothecin resistence in vitro" Jpn J Cancer Res. 88. 1211-1217 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] WATAMABE, H.: "Strain differences in induction and modification of stomach tumor developmetnt in mice treated with X-irradiation" J Toxicol Pathol. 10. 211-215 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] OGURI, Y.: "Expression of γ-glutamylcysteine synthetase (γ-GCS) and multidrug resistance-associated protein (MRP) but not human canalicular multispecific organic anion transporter (cMOAT), genes correlates with exposure of human lung cancers to platimum drugs" Br J Cancer. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] SHOJI, S.: "Correlation of developmental malformations and intrauterine deaths in γ-ray-irradiated scid mice embryos" Int J Rad Biol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawaishi, K.et al.: "Decreased L-selectin expression in CD34-positive cells from patients with chronic myelocytic leukemia." Br.J.Haematol.93. 367-374 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohashi, N.et al.: "No alterlation in DNA topoisomerase 1 gene related to CPT-11 resistance in human lung cancer." Jap.J.Cancer Res.87. 1280-1287 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kimura, A.et al.: "c-kit point mutation in patients with myeloproliferative disorders." Leukemia and Lymphoma. 25. 281-287 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morita, E.et al.: "A novel point mutation in the steroid sulfatase gene in x-linked ichthyosis." J.Invest.Dermatol.109. 244-245 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi, T.et al.: "The role of glucuronidation in 7-Ethyl-10-hydroxycamptothecin resistence in vitro." Jpn.J.Cancer Res.88. 1211-1217 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Oguri, Y.et al.: "Expression of gamma -glutamylcysteine synthetase (gamma -GCS) and multidrug resistance-associated protein (MPR) but not human canalicular multispecific organic anion transporter (cMOAT), genes correlates with exposure of human lung cancers to platinum drugs." Br.J.Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] NAKAGAWA,Y.: "Colon tissue implanted into the glandular stomach in rats lack susceptibility to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) carcinogenesis" Oncology Reports. 4. 517-519 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] WATANABE,H.: "Induction of insulinomas and kidney tumors in SD male rats by x-irradiation of gastric region" J Toxicol Pathol. 10. 77-81 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] KIMURA,A.: "c-kit point mutation in patients with myeloproliferative disorders" Leukemia and Lymphoma. 25. 281-287 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] MORITA,E.: "A novel point mutation in the steroid sulfatase gene in x-linked ichthyosis" J Invest Dermatol. 109. 244-245 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] TAKAHASHI,T.: "The role of glucuronidation in 7-Ethyl-10-hydroxycamptothecin resistence in vitro" Jpn J Cancer Res. 88. 1211-1217 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] WATAMABE,H.: "Strain differences in induction and modification of stomach tumor development in mice treated with X-irradiation" J Toxicol Pathol. 10. 211-215 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] OGURI,Y.: "Expression of γ-glutamylcysteine synthetase (γ-GCS) and multidrug resistance-associated protein (MRP). but not human canalicular multispecific organic anion transporter (cMOAT), genes correlates with exposure of human lung cancers to platinum drugs." Br J Cancer. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] SHOJI,S.: "Correlation of developmental malformations and intrauterine deaths in γ-ray-irradiated scid mice embryos" Int J Rad Biol. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 加藤 修: "γ線誘導アポトーシスに対して修飾効果を有する細胞増殖因子の検討" 広島医学. 49. 441-443 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kawaishi, K.: "Decreased L-selectin expression in CD34-positive cells from patients with chronic myelocytic leukemia" British Journal of Haematology. 93. 367-374 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohashi, N.: "No alterlation in DNA topoisomerase 1 gene related to CPT-11 resistance in human lung cancer" Japanese Journal of Cancer Research. 87. 1280-1287 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 加藤 修: "細胞増殖因子のガンマ線誘導アポトーシス制御機構について" 長崎医学会雑誌. 71. 329-331 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakagawa, Y.: "Colon tissue omplanted into the glandular stomach in rats lack susceptibility to N-methy-N′-nitro-N-nitrosoguanidine (MNNG) carcinogenesis" Oncology Reports. in press. (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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