| Project/Area Number |
09470003
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including Histology/Embryology)
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
HIROSAWA Kazushige The University of Tokyo, Inst Med Sci, Professor, 医科学研究所, 教授 (30009980)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAGARA Hiroshi The University of Tokyo, Inst Med Sci, Research Assoc., 医科学研究所, 助手 (50145041)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
|---|
| Keywords | Iris / Monoclonal Antibody / Pigment Cell / Capillary / HRP |
|---|
| Research Abstract |
1. Monoclonal antibodies against chick iris and ciliary body :
Iris and ciliary bodies with their appendages were dissected from chick eye balls and were processed for rainsing of monoclonal antibody according to the method developed in our laboratory (ref. Exp Eye Res 67,1998).
A variety of antibodies were produced in the mice used for the study. Staining patterns were categorized into 14. Among the antibodies raised, 9 antibodies were established as monoclonal antibodies. Their characterisation is being analyzed.
2. Overview of vessels in the iris :
Arterioles, venules and capillaries were found in the iris stroma. Among them, capillaries were most easily identified and frequently encountered. The capillaries had unfenestrated endothelial cells, between which were sealed with tight junctions. Capillary endothelial cells were surrounded with thick basal lamina and thick collagen fibriles.
3. On the permeability of endothelial cells in ciliary muscles and iris of monkey :
Capillary endothelial cells in the ciliary muscles are structually different from those of the ciliary processes in that they are unfenestrated. From this point of view, both ciliary muscles and iris endothelial cells were used to study the permeability of horse radish peroxidase(HRP)to the unfenestrated endothelial cells. In brief, HRP injected into the anterior chamber entered the iris stroma, cell bodies of endothelial cells and into the lumen of capillaries in 15 min. On the contrary, HRP injected into the venular system stayed in the capillary lumen and the cell bodies even after 30min. This result is consistent with results reported so far.
|
