| Project/Area Number |
09557108
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Thoracic surgery
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| Research Institution | Gifu University |
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Principal Investigator |
HIROSE Hajime Gifu Univ.School of Medicine, Professor, 医学部, 教授 (20101272)
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| Co-Investigator(Kenkyū-buntansha) |
IWATA Hisashi Gifu Univ., School of Medicine, Instructor, 医学部, 助手 (90303495)
YASUDA Hiroyuki Gifu Univ., School of Medicine, Instructor, 医学部・附属病院, 助手 (50293577)
MORI Yoshio Gifu Univ., School of Medicine, Assistant Professor, 医学部・附属病院, 講師 (40220032)
二村 直樹 岐阜大学, 医学部・附属病院, 医員
村川 真司 岐阜大学, 医学部・附属病院, 助手 (40229977)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥11,800,000 (Direct Cost: ¥11,800,000)
Fiscal Year 1998: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1997: ¥7,900,000 (Direct Cost: ¥7,900,000)
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| Keywords | endothelial cell / coronary artery bypass grafting / C-type natriuretic peptide / CD34 positive progenitor endothelial cell / C-type natriuretic peptide / 遺伝子治療 / リポソーム法 / グラフト内膜肥厚 |
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| Research Abstract |
It is an important issue to regulate the occlusion. of vein grafts after coronary artery bypass grafting (CABG) for ischemic heart disease. We investigated how the endothelial cell proliferation in vein grafts caused the occlusion of vein grafts using peripheral progenitor endothelial cells. Progenitor endothelial cells were obtained from human peripheral blood using the anti-CD34 antibody-coated magnet beads method. We observed that progenitor endothelial cells developed to endothelial cells in the culture with bovine brain extract, which was endothelial growth factor.
Conversely, we measured c-type natriuretic peptide (CNP), which inhibited endothelial cell proliferation as well as one of urine progressing peptide family, In the patients for CABG, CNP increased only during extracorponeal circulation. When we utilize constitutive express CNP, there are possibility to prevent the occlusion of vein grafts.
Therefore, We have the next issue to analyze the relationship between progenitor endothelial cells and CNP.In addition, we are going to investigate whether or not CNP gene therapy will be useful to prevent the endothelial cell proliferation in venus graft.
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