| Project/Area Number |
09670452
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Keio University |
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Principal Investigator |
YANAGIDA Junichi Keio University, Legal Medicine, School of Medicine, Professor, 医学部・法医学, 教授 (70049790)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Masaaki The Saitama Medical School, Legal Medicine, Research Assistant, 法医学, 助手 (50129160)
MURAI Tatsuya Keio University, Legal Medicine, School of Medicine, Associate Professor, 医学部・法医学, 助教授 (80129692)
SHINOZUKA Tatsuo Keio University, Legal Medicine, School of Medicine, Assistant Professor, 医学部・法医学, 講師 (70095610)
TOMITA Akiyo Keio University, Legal Medicine, School of Medicine, Research Assistant, 医学部・法医学, 助手 (10286445)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | HLA class II gene / DNA polymorphism / paternity testing / direct PCR |
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| Research Abstract |
1) HLA DQA 1 and HLA DRB genotypings by the PCR-SSP and PCR-SSO methods were applied to the cases of disputed paternity. Conventional blood group testing and HLA class II genotyping (HLA DQA 1 and HLA DRB) were good agreement with the caes of paternity test.
2) In field of forensic medicine, biological samples such as bloodstain, semen, and hair are very important samples for personal identification. It has to spend much time and make many effects to extract DNA from torensic samples. The Drop PCR Kit for human blood, by which PCR reaction directly carried out on whole blood and bloodstain without DNA extraction process, was applied to the analyses of HLA DQα and HLA DRB geontypings. The experimental results suggested that HLA class II genotyping from small amount or bloodstain (size: 1mm x 1mm) by direct PCR could be applied to forensic practices.
3) The DNA extracted from the nails of human living bodies and cadavers was examined to clarify its usefulness in the practical analysis of DNA polymorphisms. No significant differences were shown in the recovery and the purity of five different methods (phenol-chloroform) method, SMITEST DNA extraction kit, Wako DNA Extractor WB Kit, DnaQuick and SepaGene) for DNA extraction from nails of living bodies. The DNA from cadaveric nails was able to be extracted by phenol-chloroform method. The applicability of the human nail DNA for the polymorphism analyses with HLA DQ α typing, HLA-DRB typing and PM-PCR typing suggested that the DNA extracted from human nails (both living bodies' and cadavers') is useful the practical test in forensic science.
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