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Analysis of gene related with the epileptogenesis of inherited epileptic model

Research Project

Project/Area Number 09671000
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionOsaka City University

Principal Investigator

MUI Koji  Osaka City University Medical School, Lecturer, 医学部, 講師 (20219835)

Co-Investigator(Kenkyū-buntansha) KATSUMOTO Eiichi  Osaka City University Medical School, Research Associate, 医学部, 助手 (90271189)
KIOKA Tetsuro  Osaka City University Medical School, Lecturer, 医学部, 講師 (40254396)
YAMAGAMI Sakae  Osaka City University Medical School, Professor, 医学部, 名誉教授 (20047004)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsEL mice / Epilepsy / RAP-PCR / Seizure / Epileptogenesis / Elマウス / SEIZURE / EPIIEPTOGENESIS / mRNA
Research Abstract

In present study we tried to identify the gene appeared on the process of the epileptogenesis in the EL mouse that is a model for human regional related epilepsy by RNA arbitrarily primed PCR method.
We got twenty PCR products existing in loss-up stimulated and seizure experienced EL mouse (EL [s]) brain but not existing in seizure unexperienced EL mouse (EL[ns]) brain at 100 days of age. After second PCR products were subcloned to the pBSK II vector, we determined partial sequence, followed by researched the homology with already known genes. Six clones were unknown genes and three clones were mitochondrial DNAs and two clones were genomic DNAs. Nine clones were unknown of function or have not cleared relating with epilepsy or seizure, although they were already known genes. We tried to analyze fifteen clones by Northen blotting, but only two clones hybridized with mRNA from both El mice brains without significance. Remaining thirteen clones did not hybridize with mRNA from both mice by Northen blotting or RNA protection assay. Using quantitative RT-PCR, twelve clones were not significant different between EL [s] and EL [ns] mice. Only one clone, that was homologous with pipin protein over 98%, was clearly more abundant in EL [s] mice brain that EL [ns]. Since pinin is a cell junction-associated protein with a little understanding about its distribution in brain and relationship with epilepsy or seizure, we are investigating in histochemistory and comparing with both EL mouse brains.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] T. Kioka, S. Yamagami, K. Mui and H. Onishi: "Neclear polyadenylate polymerase activity in the brain of seizure-prone El mice"Paychiatr. Clin. Neurosci.. 51. 151-155 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 原田智行、撫井弘二、木岡哲郎、勝元栄一、古塚大介、山上榮: "GABAニューロンの遺伝子発現に及ぼす柴朴湯の影響"大阪精神神経科漢方研究会誌. 2. 35-38 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Ozaki, E. Katsumoto, K. Mui, D. Furutsuka and S. Yamagami: "Distribution of Fos- and Jun-related proteins and activator protein-1 composite factors in mouse brain induced by neuroleptics"Neuroscience. 84(4). 1187-1196 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 黒田陽子、撫井弘二、中西亜紀、古塚大介、大西博、山上榮: "自然発症てんかんマウス脳の蛋白質合成系におよぼす発作の影響"大阪てんかん研究会雑誌. 9(1). 1-9 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 尾崎宣洋、勝元栄一、撫井弘二、山上榮: "抗精神病薬によって誘導される Nerve Growth Factorのin situ hybridization法と免疫組織学的方法による検討"精神薬療基金研究年報. 31. 50-57 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Ozaki, K. Mui, S. Yamagami: "Comparison of the effects of dopamine D1 and D2 receptor antagonists on nerve growth factor mRNA expression"Eur. J. Pharmacol.. 369. 133-143 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Ozaki, E.Katsumoto, K.Mui and S.Yamagami: "Neuroleptics induce the expression of nerve growth factor : an in situ hybridization and immunohistological study"Ann.Rsp.Pharmacopsychiat.Res.Found.(in Japanese). 31. 50-57 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Ozaki, K.Mui and S.Yamagami: "Comparison of the effects of dopamine DィイD21ィエD2 and DィイD22ィエD2 receptor antagonists on nerve growth factor mRNA expression"Eur.J.Pharmcol. 369. 133-143 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y.Umemoto, K.Mui, A.Nakanishi, T.kioka and S.Yamagami: "Translational activity in seizure-susceptible EL mouse brain"Osaka J.Epilepsy Res. (in Japanese). 10. 1-9 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Ozaki, E.Katsumoto, K.Mui, D.Furutsuka and S.Yamagami: "Distribution of Fos- and Jun- related proteins and activator protein -1 composite factors in mouse brain induced by neuroleptics"Neurosci. 84. 1187-1196 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Ozaki, E.Ktsumoto, T.Harada, K.Mui and S.Yamagami: "Distribution of c-fos and c-jun mRNAs, and BDNF mRNA regarding mouse brain induced by neuroleptics : in situ hybridization studies"Ann.Rsp.Pharmacopsychiat.Res.Found. (in Japanese). 29. 41-48 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Harada, K.Mui, T.Kioka, E.Katsumoto, D.Furutsuka and S.Yamagami: "Effect of Saiboku-to on gene expression of GABAnergic neuron"Osaka J.Kanpo Res. (in Japanese). 2. 35-38 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T.Koida, S.Yamagami, K.Mui and H.Onishi: "Nuclear polyadenylate polymerase activity in the brain of seizure-prone EL mice"Psychiatr. Clin. Neurosci.. 51. 151-155 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] T. Kioka, S. Yamagami, K. Mui and H. Onishi: "Nuclear polyadenylate polymerase activity in the brain of seizure-prone El mice"Psychiatr. Clin. Neurosci.. 51. 151-155 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] 原田智行、撫井弘二、木岡哲郎 勝元栄一、古塚大介、山上榮: "GABAニューロンの遺伝子発現に及ぼす柴朴湯の影響"大阪精神神経科漢方研究会誌. 2. 35-38 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Ozaki, E. Katsumoto, K. Mui, D. Furutsuka and S. Yamagami: "Distribution of Fos- and Jun-related proteins and activator protein-1 composite factors in mouse brain induced by neuroleptics"Neuroscience. 84・4. 1187-1196 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 黒田陽子、撫井弘二、中西亜紀、古塚大介、大西博、山上榮: "自然発症てんかんマウス脳の蛋白質合成系におよぼす発作の影響"大阪てんかん研究会雑誌. 9・1. 1-9 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] 尾崎宣洋、勝元栄一、撫井弘二 山上栄: "抗精神病薬によって誘導されるNerve Growth Factorのin situ hybridization法と免役組織学的方法による検討"精神薬療基金研究年報. 31. 50-57 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Ozaki, K. Mui, S. Yamagami: "Comparison of the effects of dopamine D1 and D2 receptor antagonists on nerve growth factor mRNA expression"Eur. J. Pharmacol.. 369. 133-143 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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