Gas mediators and fibrinolytic factors in the mechanism on osteoarthritis
Project/Area Number |
09671527
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Orthopaedic surgery
|
Research Institution | KINKI UNIVERSITY SCHOOL OF MEDICINE |
Principal Investigator |
KIKUCHI Hiraku KINKI UNIV., LECTURE, 医学部, 講師 (10195208)
|
Project Period (FY) |
1997 – 1998
|
Project Status |
Completed (Fiscal Year 1998)
|
Budget Amount *help |
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
|
Keywords | osteoarthritis / gas mediator / fibrinolytic factors / mechanical stress / 軟骨組織 / メカニカルストレス / 線溶 / ガスメディエーター |
Research Abstract |
(1)The effect of static compression on the release of superoxide (SO) and nitric oxide (NO) from cartilage obtained from rabbit knee joints with tissue defects was studied. A static pressue of 5kg for 10min. applied to the cartilage obtained from the area surrounding the defect groups compered with the perichondrial graft groups released sugnificant amounts of SO and NO during 1 day to 3 weeks after operation. Histrogically, cartilage was regenerated in the graft groups 3 week after operation, although only a fibrous tissue filled the defects in the defects groups. (2)The amount of urokinase type plasminogen activator (uPA) and matrix metalloprotease (MMP)-1, 3 increased in human cartilage tissue from osteoarthritis (OA), although the PA inhibitor-1 (PAI-1)and tissue inhibitor of MMP decreased compared to amputated control cartilage. The uPA content was higher at the weight-bearing site in OA patients compared to cartilage tissue from controls. The PAI-1 content was higher in osteophyte-forming sites compared to controls. The levels of uPA and PAI-1 antigen increased in cartilage tissues exposed to mechanical stress. (3)The release of uPA and PAI-1 antigens from specimens was suppressed with high molecular weight hyaluronic acid (HA). (4)Intraarticular of HA reduces fibrinolytic activity in joint space. it is protective against joint destruction caused by the serine-metalloprotease cascade.
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Report
(3 results)
Research Products
(6 results)