| Project/Area Number |
09671943
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
SHOJI Shigeru Tohoku University, Dental Hospital, Lecturer, 歯学部・附属病院, 講師 (10142986)
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| Co-Investigator(Kenkyū-buntansha) |
NEMOTO Eiji Tohoku University School of Dentistry, Assistant, 歯学部, 助手 (40292221)
IIYAMA Masao Tohoku University School of Dentistry, Assistant, 歯学部, 助手 (00193152)
HORIUCHI Hiroshi Tohoku University School of Dentistry, Professor, 歯学部, 教授 (00013962)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Discrimination / cementoblast / odontoblast |
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| Research Abstract |
To regenerate the periodontal tissue, many researches have been done. The guided tissue regeneration method attaches the membrane to a root, and makes the regenerationspace for cementum, periodontal ligament and alveolar bone. Recently the enamel protein(amelogenin), which is extracted from pig toothgerm, is applied to induce the cementoblast.
In 1997 we thought that the cementoblast secrete non-collagenous protein (osteopontin, sialoprotein ). So if we found the cells near the non-collagenous protein stained byimmuno-histochemical technique, we thought those cells were cementoblast. But it was very difficult to determine the cells only by their position.
In 1998 we fixed our eyes on Hertwig's epithelial sheath. At the position of the formation of tooth root, a mass of Hertwigh's epithelial cells contacts with the mesenchymal cells through the laminin. We thought these mesenchymal cells, whose have a vitamin D3receptor, were cementoblast. The observation using translating electron microscopy showed the cells contacted with Hertwig's epithelial cells, and that these cells secreted the alkalinephosphatase. But we could not find the vitamin D3 receptor on these cells.
We should reconsider the criteria of cementoblast, and pay the attention to the difference of cementum between human and rat.
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