Identification of factirs involved in formation of the Drosophila germ line
| Project/Area Number |
09680712
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Aichi Cancer Center |
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Principal Investigator |
MATSUKAGE Akio Aichi Cancer Center, Laboratory of Cell Biology, Chief, 生物学部, 部長 (90019571)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGISHI Masahiro National Institute of Bioscience and Human Technology, Genome Analysis Laborator, 生命工学技術研究所, 主任研究官 (00220252)
YAMAGUCHI Masamitsu Aichi Cancer Center, Laboratory of Cell Biology, Section Head, 生物学部, 室長 (00182460)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Germ line cells / Droshophila / differential display method / differential display法 / 生殖系列 / 遺伝子発現 / cDNAクローニング |
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| Research Abstract |
The puropose of the present study is to idenfity and characterize factors invovlved in the formation of germline cells and morphology. The study has been started by seraching mRNA species which decreas or increase in speficic mutant lines lacking germline formation. By using the differntial display method, we have isolated 23 cantidate genes out of which four are identical to previously reported genes while others are novel genes. In situ hybridization demonstrated that trasncripts of all these genes do not localize in the site for the germ line formation. Previously reported genes are :
similar to uridine kinase
yeast SCOI protein
yeast CBF5/rat nucleolar protein NAP57
Drosophila three rows protein
Further analysis is required with novel islated genes.
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Report
(3 results)
Research Products
(20 results)
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[Publications] Sawado, T., Hirose, F., Takahashi, Y., Sasaki, T., Shinomiya, T., Sakaguchi, K., Matsukage, A.and Yamaguchi, M.: "The DNA replication-related element (DRE)/DRE-binding factor system is a transcriptiona regulator of the Drosophila E2F gene" J.Biol.Chem.273. 26042-26051 (1998)
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Publications] Sawado, T., Yamaguchi, M., Nishimoto, Y., Ohno, K., Sakaguchi, K.and Matsukage, A.: "dE2F,a novel E2F-family transcription factor in Drosophila melanogaster" Biochem.Biophys.Res.Commun.251. 409-415 (1998)
Description
「研究成果報告書概要(欧文)」より
Related Report
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