1998 Fiscal Year Final Research Report Summary
Identification of factirs involved in formation of the Drosophila germ line
| Project/Area Number |
09680712
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Aichi Cancer Center |
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Principal Investigator |
MATSUKAGE Akio Aichi Cancer Center, Laboratory of Cell Biology, Chief, 生物学部, 部長 (90019571)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGISHI Masahiro National Institute of Bioscience and Human Technology, Genome Analysis Laborator, 生命工学技術研究所, 主任研究官 (00220252)
YAMAGUCHI Masamitsu Aichi Cancer Center, Laboratory of Cell Biology, Section Head, 生物学部, 室長 (00182460)
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| Project Period (FY) |
1997 – 1998
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| Keywords | Germ line cells / Droshophila / differential display method |
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| Research Abstract |
The puropose of the present study is to idenfity and characterize factors invovlved in the formation of germline cells and morphology. The study has been started by seraching mRNA species which decreas or increase in speficic mutant lines lacking germline formation. By using the differntial display method, we have isolated 23 cantidate genes out of which four are identical to previously reported genes while others are novel genes. In situ hybridization demonstrated that trasncripts of all these genes do not localize in the site for the germ line formation. Previously reported genes are :
similar to uridine kinase
yeast SCOI protein
yeast CBF5/rat nucleolar protein NAP57
Drosophila three rows protein
Further analysis is required with novel islated genes.
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Research Products
(12 results)
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[Publications] Sawado, T., Hirose, F., Takahashi, Y., Sasaki, T., Shinomiya, T., Sakaguchi, K., Matsukage, A.and Yamaguchi, M.: "The DNA replication-related element (DRE)/DRE-binding factor system is a transcriptiona regulator of the Drosophila E2F gene" J.Biol.Chem.273. 26042-26051 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sawado, T., Yamaguchi, M., Nishimoto, Y., Ohno, K., Sakaguchi, K.and Matsukage, A.: "dE2F,a novel E2F-family transcription factor in Drosophila melanogaster" Biochem.Biophys.Res.Commun.251. 409-415 (1998)
Description
「研究成果報告書概要(欧文)」より
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