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Resistance of Insect to Pathogenic Viruses

Research Project

Project/Area Number 10556012
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section展開研究
Research Field 蚕糸・昆虫利用学
Research InstitutionThe University of Tokyo

Principal Investigator

NAGATA Masao  University of Tokyo, Graduate School of Frontier Science, Professor, 大学院・新領域創成科学研究科, 教授 (70107407)

Co-Investigator(Kenkyū-buntansha) OZAKI Masataka  University of Tokyo, Graduate School of Agricultural and Life Science, Assistant Professor, 大学院・農学生命科学研究科, 助手 (00270893)
SHIMADA Tohru  University of Tokyo, Graduate School of Agricultural and Life Science, Associate Professor, 大学院・農学生命科学研究科, 助教授 (20202111)
KOBAYASHI Masahiko  University of Tokyo, Graduate School of Agricultural and Life Science, Professor, 大学院・農学生命科学研究科, 教授 (60162020)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,300,000 (Direct Cost: ¥13,300,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥10,300,000 (Direct Cost: ¥10,300,000)
KeywordsInsect / Defense system / 発育抵抗性 / カイコ血液 / アポトーシス
Research Abstract

Nucleopolyhedrovirus (NPV) of the silkworm was inactivated after incubation with the silkworm hemolymph. Inactivation activity was located in the fraction that passed a filter with 5 kd M.W.and inhibitors of that activity existed in the fraction with more than 5Kd. Inactivation activity was retained after heat and protease treatment of the fraction. We also detected that glutathion inactivated NPV and are analyzing inactivation mechanism of NPV by the hemolymph and other compounds.
An increase in median lethal dose (LD50) and median lethal time (LT50) values with increased larval age was recorded in bioassay studies of the nucleopolyhedrovirus (NPV) infected Spodoptera litura Fab. larvae. The LD50 values and LT50 increase with increased larval age. Larvae those were more than 11-day-old after hatching was not infected through oral inoculation even at the highest dose of the trial. However, intrahaemocoelic injection of NPV into matured larvae resulted into death due to viral infection indicating a lack of susceptibility possibly due to gut mediated barrier that developed as the larvae approached pupation.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Katsuma,S. et al.: "Molecular characterization of baculovirus Bombyx mori nucleopolyhedrovirus polyhedron mutants"Archi.Virol.. 144. 1275-1285 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Monobrullah,MD.and Nagata,M.: "Immunity of lepidopteran insects against baculoviruses"J.ent.Res. 23. 185-194 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Monobrullah,MD.and Nagata,M.: "Effects of larval age on susceptibility of Spodoptera litura (Lepidoptera : Noctuidae) to Spodoptera litura multiple nuclear polyhedrosis virus"Canadian Entomologist. 132. 337-340 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsuma, S.et al.: "Molecular characterization of baculovirus Bombyx mori nucleopolyhedrovirus polyhedron mutants"Archi.Virol.. 144. 1275-1285 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Monobrullah, MD.and Nagata, M.: "Immunity of lepidopteran insects against baculoviruses"J.ent.Res.. 23. 185-194 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Monobrullah, MD.and Nagta, M.: "Effect of larval age on susceptibility of Spodoptera litura (Lepidoptera : Noctuidae) to Spodoptera litura multiple nuclear polyhedrosis virus"Canadian Entomologist. 132. 337-340 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Monobrullah,MD.and Nagata,M.: "Effect of larval age on susceptibility of Spodoptera litura (Lepidoptera : Noctuidae) to Spodoptera litura multiple nuclear polyhedrosis virus"Canadian Entomologist. 132. 337-340 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Katsuma,S: "Molecular characterization of baculovirus Bombyx mori nucleopolyhedrovirus polyhedron mutants"Archives Virology. 144. 1275-1285 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki,M.G.et.al.: "Bm kettin,ahomologue of the Drosophila kettin gene,is located on the Z chromosome inBombyx mori and is not dosage compensated." Heredity. 82. 170-179 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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