| Project/Area Number |
10671145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Fukuoka University |
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Principal Investigator |
YASUNAMI Yohichi Fukuoka Univ., Sch. Medicine, Assoc. Prof., 医学部, 助教授 (00166521)
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| Co-Investigator(Kenkyū-buntansha) |
白石 君男 福岡大学, 医学部, 助手 (90187518)
加藤 寿彦 福岡大学, 医学部, 教授 (80078766)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Islet transplantation / NKT cells / Rejection / Tolerance / xenotransplantation / Anti-CD4 antibody |
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| Research Abstract |
Pancreatic islet transplantation represents a potential treatment for insulin-dependent diabetes mellitus. However. the precise cellular and molecular mechanisms of the immune reactions against allogeneic and xenogeneic transplanted islets remain nuclear. In the present study, we demonstrate that CD4a+ Vα l4 NKT cells, a distinct lymphoid cell lineage recently identified, are required for the acceptance of intrahepatic rat islet xenografts. Rat islet xenografts were accepted by C57BL/6 mice when certain doses of anti-CD4mAb were administrated after transplantation. No immunosuppressive drug is required in this procedure. The dose or anti-CD4mAb was critical, and the beneficial effect appeared to be associated with reappearance of CD4 + NKT cells around 14 days after transplantation. The anti-CD4mAb-mediated acceptance of rat islet xenografts was not observed in Vα 14 NKT cell-deficient mice, where essentially no Vα 14 NKT cells exist and conventional lymphoid cells remain intact. Moreover, the adoptive transfer of Vα 14 NKT cells into Vα 14 NKT cell-deficient mice reconstituted acceptance of rat islet xenografts. These results indicate that Vα 14 NKT cells play a crucial role in the acceptance of rat islet xenografts in mice treated with anti-CD4 antibody.
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