| Project/Area Number |
10680141
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
食生活
|
|---|
| Research Institution | Ochanomizu University |
|---|
Principal Investigator |
MURATA Masatsune OCHANOMIZU UNIVERSITY, DEPARTMENT OF NUTIRTION AND FOOD SCIENCE, ASSOCIATE PROFESSOR, 人間文化研究科, 助教授 (60210051)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HONNMA Seiichi OCHNOMIZU UNIVERSITY, DEPARTMENT OF NUTIRTION AND FOOD SCIENCE, PROFESSOR, 生活科学部, 教授 (50017240)
|
|---|
| Project Period (FY) |
1998 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | transgenic plant / protein expression / Agrobacterium / tobacco / apple / phenolic / tobacco |
|---|
| Research Abstract |
One of the issues on genetically modified foods is that unexpected or unintended proteins or metabolites will occur as a result of the genetic modification of crops. Here we have prepared transgenic tobacco and apple shoots as model plants and analyze the proteins and phenolics to give a fundamental knowledge on the safety of the genetically modified foods An antibiotic-resistant gene was used as a maker for the selection of transgenic plants. Beta-glucuronidase gene was used to colorimetrically examine the expression of inserted gene. Proteins and phenolics of transgenic shoots were compared with those of non-transgenic shoots. Proteins and phenolics were analyzed by SDS-PAGE and HPLC, respectively. Consequently, there were no significant differences between them.
|
