| Project/Area Number |
10680563
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Niigata University |
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Principal Investigator |
ARAKI Shigeko Brain Research Institute, Niigata University Research Associate, 脳研究所, 助手 (70018604)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Sachiko Brain Research Institute, Niigata University Research Associate, 脳研究所, 助手 (60018603)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | structural analysis / Aplysia / phosphonoglycosphingolipid / egg mass / lectin / phosphonoglycolipid / Aplysia kuroda / Aplysia kurodai |
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| Research Abstract |
We isolated a novel glycolipid, EGL-II, in the egg of Aplysia kurodai and identified its structure as follows.
(1) Amino acid analysis showed the presence of 2-AEPn and glucosamine in a molar ratio of 3 : 1.
(2) The major aliphatic components of ceramide were palmitic acid, stearic acid and anteisononadeca-4-sphingenine.
(3) EGL-II was composed of glucose/galactose/N-acetylglucosamine/3-O-methylgalactose in a molar ratio of 1 : 5 : 1 : 1 and 2-AEPn was attached to each of 2 mol of galactose and 1 mol of glucose.
(4) From the results of the permethylation studies and the fragmentation diagram of the FAB-MS and SIMS spectra, the sugar sequence and linkage of EGL-II was concluded to be.
<<chemical formula>>
Gal1-3(GlcNAc1-2)Gal1-3(3-O-MeGal1-2)Gal1-3(Gal1-2)Gal1-4Glc1--Cer
(5) The proton magnetic resonance data revealed β-Glc, β-Gal, α-GlcNAc, α-3-O-MeGal, and four α-Gal protons in the anomeric region.
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