| Project/Area Number |
11138210
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas (A)
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| Allocation Type | Single-year Grants |
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| Research Institution | Institute for Microbial Disease, Osaka Univ. |
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Principal Investigator |
SUZUKI Akira (Inst.Microbial Disease, Osaka Univ., Assist Prof.), 微生物病研究所, 講師 (10311565)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUMOTO Manabu (Inst.Dev.Aging and Cancer, Tohoku Univ., Professor.), 加齢医学研究所, 教授 (60156809)
仲野 徹 大阪大学, 微生物病研究所, 教授 (00172370)
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| Project Period (FY) |
1999
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1999: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | PTEN / Brain Tumor / Cancer Suppressor Gene / Oncogenesis |
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| Research Abstract |
1) Targeting vector was designed by inserting the exon4-5, including the phosphatase domain, between loxP sites to generate the conditional KO mice of PIEN.PTENflox/+ mice were obtained by injecting the PTENflox/+ ES cells into the blastocysts.
2) PTENflox/+ mice were crossed with Lck-Cre transgenic mice to delete PTEN gene in T cells. Ninty-five % of thymocytes were deleted PTEN at both DNA and protein level and this mutation is a null-mutation, confirming that Cre-loxP system worked well in vivo. All of the Lck-CrePTENflox/- mice developed into CD4 T cell lymphoma/leukemias in 17 weeks after birth.
3) To delete the PTEN gene specifically in brain, we are now crossing the PTENflox/+ mice with GFAP-Cre and MBP-Cre transgenic mice to generate GFAP-CrePTENflox/- and MBP-CrePTENflox/- mice.
4) PTEN+/- mice were observed to see the spectrum of suffering cancers. We found endometrial cancer and breast cancer were also seen in a longer observation period, although glioma has never been observed.
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