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Regulative Mechanisms and Manipulation of the Germ Cell Lineage

Research Project

Project/Area Number 11234101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NAKATSUJI Norio  Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (80237312)

Co-Investigator(Kenkyū-buntansha) NAGAHAMA Yoshitaka  Institute for Basic Biology, Professor, 基礎生物学研究所, 教授 (50113428)
KATSUKI Motoya  Institute for Basic Biology, Director, 基礎生物学研究所, 所長 (20051732)
NISHIMUNE Yoshitake  Osaka University, Institute for Medical Microbiology, Professor, 微生物病研究所, 教授 (80029793)
YAMAMURA Kenichi  Kumamoto University Medical School, Professor, 医学部, 教授 (90115197)
TSUNODA Yukio  Kinki University, Faculty of Agriculture, Professor, 農学部, 教授 (80217364)
帯刀 益夫  東北大学, 加齢医学研究所, 教授 (10099971)
本庶 佑  京都大学, 大学院・医学研究科, 教授 (80090504)
Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2002: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsGerm cells / mammalian / embryo manipulation / germ lineage
Research Abstract

This research group was aimed at facilitating and encouraging scientific interactions and collaboration among the scientists involved in this priority area. For such purpose, this group organized annual meeting of the priority area, and also symposia open to general scientists. The most significant was the international symposium on "Development and Epigenetics of Mammalian Germ Cells and Pluripotent Stem Cells, "held on 19-21 November, 2001 in Kyoto, with more than 300 participants and 25 speakers.
Speakers includes : Anne McLaren (Cambridge, UK) "Germ cells and stem cells : the key to developmental potency"/Patrick Tam (Sydney, Australia) "Allocation and differentiation of the progenitors for germ cells and urogenital tissues"/Austin Smith (Edinburgh, UK) "Signalling self-renewal in pluripotent stem cells"/Guang-Quan Zhao (Dallas, USA) "BMP signaling in the generation of primordial germ cells in the mouse"/Chris Wylie (Cincinnati, USA)"Migration of mouse primordial germ cells"/Robin L … More ovell-Badge (London, UK)"The Molecular Genetics of Sex Determination in Mammals : Building Pathways"/Azim Surani (Cambridge, UK)"The origin and properties of the mouse germ line."/N. B. Hecht (Philadelphia, USA)"Selective intracellular and intercellular mRNA transport in mammalian germ cells"/Hitoshi Niwa (Center for Developmental Biology, RIKEN, Japan) /Yukio Tsunoda (Kinki Univ., Japan)/Nobuo Takagi (Hokkaido Univ., Japan)/Hiroyuki Sasaki (Nat. Inst. of Genetics, Japan)/Fumitoshi Ishino (Tokyo Inst. for Technology, Japan)"Genomic imprinting in the germ cell lineage"/Takashi Tada (Kyoto Univ., Japan)/Junji Takeda (Osaka Univ., Japan)/Kuniya Abe (Kumamoto Univ., Japan), Yasuhisa Matsui (Osaka Medical Center for Maternal and Child Health, Japan), Toshiaki Noce (Mitsubishi-Kagaku Inst. of Life Sciences, Japan), Norio Nakatsuji (Kyoto Univ., Japan), Tomohiro Kono (Tokyo Univ. of Agriculture, Japan), Atsuo Ogura (Nat. Inst. of Infectious Diseases, Japan), Hiroshi Nojima (Osaka Univ., Japan), Masami Nozaki (Osaka Univ., Japan), Masaru Okabe (Osaka Univ., Japan), Gen Kondoh (Osaka Univ., Japan) Less

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (57 results)

All Other

All Publications (57 results)

  • [Publications] Kanno, Y., Tamura, M., Chuma, S., Sakurai, T., Machida, T.and Nakatsuji, N.: "A cystatin-related gene, testatin/cresp, shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiation"Int. J. Dey. Biol.. 43. 777-784 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tada, T., Obata, Y.Tada, M., Goto, Y., Nakatsuji, N., Tan, S-S., Kono, T., Takagi, N.: "Imprint switching for non-random X-chromosome inactivation during mouse oocyte growth"Development. 127. 3101-3105 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Huang, Z., Tamura, M., Sakurai, T., Chuma, S., Saito, T., Nakatsuji, N.: "In vivo transfection of testicular germ cells and transgenenis by using the mitochondrially localized jellyfish fluorescent protein gene"FEBS Lett.. 487. 248-251 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.S.Tanaka, Y.Toyooka, R.Akasu, Y Katoh-Fukui, Y.Nakahara, R.Suzuki, M.Yokoyama, T.Noce.: "Mouse homolog gene to Drosophila Vasa is required for the development of male germ cells"Genes & Dev.. 14. 841-853 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Toyooka, N.Tsunekawa, Y.Takahashi, Y.Matsui, M.Satoh, T.Noce.: "Expression and intracellular localizationof mouse Vasa-homolog protein during germ cell development"Mech. Dev.. 93. 139-149 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chuma, S., Nakatsuji, N.: "Autonomous transition into meiosis of mouse fetal germ cells in vitro and its inhibition by gp 130-mediated signaling"Devel. Biol.. 229. 468-479 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakatsuji, N.and Chuma, S.: "Differentiation of mouse primordial germ cells into female or male germ cells"Int. J. Dev. Biol.. 45. 541-548 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura, M., Kanno, Y., Chuma, S., Saito, T., Nakatsuji, N.: "Pod-1/Capsulin shows a sex-and stage-dependent expression pattern in the mouse gonad development and represses expression of Ad4BP/SF-1"Mech. Devel.. 102. 135-144 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Noce, S.Okamoto, N.Tsunekawa.: "Vasa homolog genes in mammalian germ cell development"Cell Struc. & Func.. 26. 131-136 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasegawa, K., Nakatsuji, N.: "Insulators prevent transcriptional interference between two promoters in a double gene construct for transgenesis"FEBS Lett.. 520. 47-52 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto, M., Matsui, Y: "Testis-specific expression of a novel mouse defensin-like gene, Tdl"Mech. Dev.. 116. 217-221 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kono, T., Sotomaru, Y., L, Dandolo.: "Mouse parthenogenetic embryos with mono-allelic H19 expression can develop to day 17.5 of gestation."Dev. Biol.. 243. 294-300 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue, K., Kohda, T., Lee, J., Ogonuki, N., Mochida, K., Noguchi, Y., Tanemura, K., Kaneko-Ishino, T., Ishino, F., Ogura, A.: "Faithful expression of imprinted genes in cloned mice"Science. 295. 297 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanatsu-Shinohara, M., Ogura, A., Ikegawa.M., Inoue, K., Ogonuki, N., Tashiro, K., Toyokuni, S., Honjo, T., Shinohara, T.: "Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice"Proc. Natl. Acad. Sci. USA. 99. 1383-1388 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogonuki.N., Inoue, K., Yamamoto, Y., Noguchi.Y., Tanemura, K., Suzuki, O., Nakayama, H., Doi, K., Ohtomo, K., Satoh, M., Nshida, A., Ogura, A.: "Early death of mice cloned from somatic cells"Nat Genet. 30. 253-254 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka, K., Tamura, H., Tanaka, H., Katoh, M., Futamata, Y., Seki, N., Nishimune, Y, Hara, T.: "Spermatogonia-dependent expression of testicular genes in mice"Dev. Biol.. 246. 466-479 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tadokoro, Y., Yomogida, K., Ohta, H., Tohda, A., Nishimune, Y: "Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway"Mech. Dev.. 113. 29-39 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujii, T., Tamura, K., Masai, K., Tanaka, H., Nishimune, Y., Nojima, H.: "Use of stepwise subtraction to comprehensively isolate mouse genes whose transcription is upregulated during spermiogenesis"EMBO Rep.. 3(4). 367-372 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koike, K., K.Hone, H.Fukuyama, G.Kondoh, S.Nagata, J.Takeda: "Efficient biallelic mutagenesis with Crc/loxP-mediated inter-chromosomal recombination"EMBO reports. 3-5. 433-437 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanno,Y., Tamura, M., Chuma, S., Sakurai, T., Machida, T. and Nakatsuji, N.: "A cystatin-related gene, testatin/cresp, shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiation"Int. J. Dev. Biol. 43. 777-784 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tada, T., Obata, Y., Tada, M., Goto, Y., Nakatsuji, N., Tan, S.-S., Kono, T. and Takagi, N.: "Imprint switching for non-random X-chromosome inactivation during mouse oocyte growth"Development. 127. 3101-3105 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Huang, Z., Tamura, M., Sakurai, T., Chuma, S., Saito, T., and Nakatsuji, N.: "In vivo transfection of testicular germ cells and transgenenis by using the mitochondrially localized jellyfish fluorescent protein gene"FEBS Lett.. 487. 248-251 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.S.Tanaka, Y. Toyooka, R. Akasu, Y. Katoh-Fukui, Y. Nakahara, R. Suzuki, M. Yokoyama and T. Noce: "Mouse homolog gene to Drosophila Vasa is required for the development of male germ cells"Genes & Dev.. 14. 841-853 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. Toyooka, N. Tsunekawa, Y. Takahashi, Y. Matsui, M. Satoh and T. Noce.: "Expression and intracellular localization of mouse Vasa-homolog protein during germ cell development"Mech. Dev.. 93. 139-149 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chuma, S. and Nakatsuji, N.: "Autonomous transition into meiosis of mouse fetal germ cells in vitro and its inhibition by gp130-mediated signaling"Devel. Biol.. 229. 468-479 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakatsuji, N. and Chuma, S.: "Differentiation of mouse primordial germ cells into female or male germ cells"Int. J. Dev. Biol.. 45. 541-548 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura, M., Kanno, Y., Chuma, S., Saito, T, and Nakatsuji, N.: "Pod-1/Capsulin shows a sex- and stage-dependent expression pattern in the mouse gonad development and represses expression of Ad4BP/SF-l"Mech. Devel.. 102. 135-144 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Noce, S. Okamoto, and N. Tsunekawa: "Vasa homolog genes in mammalian germ cell development"Cell Struc. & Func.. 26. 131-136 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasegawa, K. and Nakatsuji, N.: "Insulators prevent transcriptional interference between two promoters in a double gene construct for transgenesis"FEBS Lett.. 520. 47-52 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto, M, and Matsui, Y.: "Testis-specific expression of a novel mouse defensin-like gene, Tdl"Mech. Dev.. 116. 217-221 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kono, T., Sotomaru, Y., L. Dandolo: "Mouse parthenogenetic embryos with mono-allelic H19 expression can develop to day 17.5 of gestation"Dev. Biol. 243. 294-300 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue, K., Kohda, T., Lee, J., Ogonuki, N., Mochida, K., Noguchi, Y., Tanemura, K., .Kaneko-Ishino, T., Ishino, K, and Ogura, A.: "Faithful expression of imprinted genes in cloned mice"Science. 295. 297 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanatsu-Shinphara, M. Ogura, A., Ikegawa, M., Inoue, K., Ogonuki, N., Tashiro, K., Toyokuni, S., Honjo, T., and Shinohara, T.: "Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice"Proc. Natl. Acad. Sci. USA. 99. 1383-1388 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ogonuki, N., Inoue, K., Yamamoto, Y,, Noguchi, Y., Tanemura, K., Suzuki, O., Nakayama, H., Doi, K., Ohtomo, K., Satoh, M., Nishida, A., and Ogura, A.: "Early death of mice cloned from somatic cells"Nat Genet. 30. 253-254 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tanaka, K., Tamura, H., Tanaka, H., Katoh, M., Futamata, Y., Seki, N.,Nishimune, Y. and Hara, T.: "Spermatogonia-dependent expression of testicular genes in mice"Dev. Biol.. 246. 466-479 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tadokoro, Y., Yomogida, K., Ohta, H., Tohda, A. and Nishimune, Y.: "Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway"Mech. Dev.. 113. 29-39 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujii, T., Tamura, K.,Masai,K., Tanaka,H., Nishimune, Y. and Nojima, H.: "Use of stepwise subtraction to comprehensively isolate mouse genes whose transcription is upregulated during spermiogenesis"EMBO Rep. 3(4). 367-372 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koike, K., K. Horie, H. Fukuyama, G. Kondoh, S. Nagata, and T. Takeda: "Efficient biallelic mutagenesis with Cre/loxP-mediated inter-chromosomal recombination"EMBO reports. 3-5. 433-437 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamamoto, M., Matsui, Y.: "Testis-specific expression of a novel mouse defensin-like gene, Tdl"Mech. Dev.. 116. 217-221 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hasegawa, K., Nakatsuji, N.: "Insulators prevent transcriptional interference between two promoters in a double gene construct for transgenesis"FEBS Lett.. 520. 47-52 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kono, T., Sotomaru, Y., L, Dandolo.: "Mouse parthenogenetic embryos with mono-allelic H19 expression can develop to day 17.5 of gestation"Dev. Biol.. 243. 294-300 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inoue, K., Kohda, T., Lee, J., Ogonuki, N., Mochida, K., Noguchi, Y., Tanemura, K., Kaneko-Ishino, T., Ishino, F., Ogura A.: "Faithful expression of imprinted genes in cloned mice"Science. 295. 297 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanatsu-Shinohara, M., Ogura, A., Ikegawa, M., Inoue, K., Ogonuki, N., Tashiro, K., Toyokuni, S., Honjo, T., Shinohara, T.: "Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice"Proc. Natl. Acad. Sci. USA. 99. 1383-1388 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ogonuki, N., Inoue, K., Yamamoto, Y., Noguchi, Y., Tanemura, K., Suzuki, O., Nakayama, H., Doi, K., Ohtomo, K., Satoh, M., Nishida, A., Ogura, A.: "Early death of mice cloned from somatic cells"Nat Genet. 30. 253-254 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tnaka, K., Tamura, H., Tanaka, H., Katoh, M., Futamata, Y., Seki, N., Nishimune, Y., Hara, T.: "Spermatogonia-dependent expression of testicular genes in mice"Dev. Biol.. 246. 466-479 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tadokoro, Y., Yomogida, K., Ohta, H., Tohda, A., Nishimune, Y.: "Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway"Mech. Dev.. 113. 29-39 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujii, T., Tamura, K., Masai, K., Tanaka, H., Nishimune, Y., Nojima, H.: "Use of stepwise subtraction to comprehensively isolate mouse genes whose transcription is upregulated during spermiogenesis"EMBO Rep.. 3(4). 367-372 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koike, K., K.Horie, H.Fukuyama, G.Kondoh, S.Nagata, J.Takeda: "Efficient biallelic mutagenesis with Cre/loxP-mediated inter-chromosomal recombination"EMBO reports. 3-5. 433-437 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tamura, M., Kanno, Y., Chuma, S., Saito, T., Nakatsuji, N.: "Pod-1/Capsulin shows a sex-and stage-dependent expression pattern in the mouse gonad development and represses expression of Ad4BP/SF-1"Mech. Devel.. 102. 135-144 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakatsuji, N., Chuma, S.: "Differentiation of mouse primordial germ cells into female or male germ cells"Int. J. Dev. Biol.. 45. 541-548 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kanno,Y.Tamura,M.,Chuma,S.,Sakurai,T.,Machida,T.and Nakatsuji,N.: "A cystatin-related gene, testatin/cresp, shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiation."Int.J.Dev.Biol.. 43. 777-784 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 中馬新一郎,中辻憲夫: "胎仔生殖系列細胞の発生と操作"遺伝子医学. 4. 264-268 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Chuma,S. and Nakatsuji,N: "Autonomous transition into meiosis of mouse fetal germ cells in vitro and its inhibition by gp130-mediated signaling. "Devel.Biol.. 229. 468-479 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nakatsuji,N. and Chuma,S.: "Differentiation of mouse primordial germ cells into female or male germ cells."Int.J.Dev.Biol.. (In Press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tamura,M.,Kanno,Y.,Chuma,S.,Wakana,S.Saito,T.,Shirayoshi,Y. and Nakatsuji,N.: "A basic helix-loop-helix transcription factor gene, nephgonadin, isolated by differen-tial screening of the male and female fetal gonads shows a sex- and stagedependent expression pattern in the gonad development."Mech.Devel.. (In Press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kanno, Y.: "A cystatin-related gene, testatin/cresp, shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiatic"International Journal of Developmental Biology. 43. 777-784 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 中憲憲夫: "始原生殖細胞"岩波講座「現代医学の基礎」. 5巻. 2-8 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2018-03-28  

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