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Nociceptin receptor-deficient mice : distribution of nociceptin precursor protein and receptor in brain and spinal cord

Research Project

Project/Area Number 11480228
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKansai Medical University

Principal Investigator

SUGIMOTO Tetsuo  Kansai Medical University, Faculty of Medicine, Professor, 医学部, 教授 (90144352)

Co-Investigator(Kenkyū-buntansha) KASE Masahiko  Kansai Medical University, Faculty of Medicine, Research Assistant, 医学部, 助手 (10309221)
HOUTANI Takeshi  Kansai Medical University, Faculty of Medicine, Lecturer, 医学部, 講師 (60241163)
OHISHI Hitoshi  Kansai Medical University, Faculty of Medicine, Lecturer, 医学部, 講師 (00252461)
SAKUMA Satoru  Kansai Medical University, Faculty of Medicine, Research Assistant, 医学部, 助手 (70309227)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥13,700,000 (Direct Cost: ¥13,700,000)
Fiscal Year 2000: ¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 1999: ¥5,900,000 (Direct Cost: ¥5,900,000)
Keywordsβ-galactosidase / gene targeting / peptide receptor system / knockout mice / nociceptin / cDNAサブトラクション法
Research Abstract

Nociceptin/orphanin FQ (N/OFQ) is an opioid-like heptadecapeptide agonist for the opioid receptor homolog, N/OFQ receptor. To explore the precise distribution of the peptide-receptor system, we examined the brain and spinal cord from the receptor-deficient mice bearing the targeted mutation (morc^<ml>), a lacZ insertional mutation in the N/OFQ receptor gene. PreproN/OFQ mRNA was detected by in situ hybridization and the N/OFQ receptor by X-gal histochemistry. The N/OFQ receptor reflected by lacZ expression was observed at high levels in the dentate gyrus, lateral septum, subparafascicular thalamic nucleus, medial preoptic area, median preoptic nucleus, ventromedial preoptic nucleus, anterior hypothalamic area, paraventricular hypothalamic nucleus, ventromedial hypothalamic nucleus, auditory brainstem nuclei, pontine dorsal tegmentum, and nucleus of the solitary tract. In situ detection of the N/OFQ receptor mRNA by digoxigenin-labeled riboprobes coupled with tyramide signal amplification in normal and wild-type mice resulted in the regional distribution paralleling the lacZ expression in these regions. PreproN/OFQ mRNA was expressed at high levels in the subparafascicular thalamic nucleus, central gray, central tegmental field, auditory brainstem nuclei, caudal spinal trigeminal nucleus, and spinal dorsal horn. Furthermore, variable levels of expression of the peptide and receptor were seen in distinct sites of the brain and spinal cord. These data indicated a correspondence of the peptide and receptor in the local distribution at limbic, hypothalamic and brainstem sites.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report

Research Products

(12 results)

All Other

All Publications (12 results)

  • [Publications] Takeshi Houtani: "Distribution of nociceptin/orphanin FQ precusor protein and receptor in brain and spinal cord : a study using in situ hybridization and X-gal histochemistry in the receptor-deficient mice."J.Comp.Neurol.. 424. 489-508 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tetsuya Shirasaki: "Spontaneous transient outward currents : modulation by nociceptin in murine dentate gyrus granule cells."Brain Research. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeshi Houtani, Miyuki Nishi, Hiroshi Takeshima, Kazuo Sato, Satoru Sakuma, Shingo Kakimoto, Teizo Ueyama, Tetsuo Noda and Tetsuo Sugimoto.: "Distribution of nociceptin/orphanin FQ precursor protein and receptor in brain and spinal cord : a study using in situ hybridization and X-gal histochemistry in the receptor-deficient mice."J.Comp.Neurol.. 424 (3). 489-508 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tetsuya Shirasaki, Takeshi Houtani, Tetsuo Sugimoto and Hiroko Matsuda.: "Spontaneous transient outward currents : modulation by nociceptin in murine dentate gyrus granule cells."Brain Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takeshi Houtani: "Distribution of nociceptin/orphanin FQ precursor protein and receptor in brain and spinal cord : a study using in situ hybridization and X-gal histochemistry in the receptor-deficient mice."J.Comp.Neurol.. 424・3. 489-508 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tetsuya Shirasaki: "Spontaneous transient outward currents : modulation by nociceptin murine dentate gyrus granule cells."Brain Research. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyuki Nishi: "RhoN,a novel small GTP-binding protein expressed predominantly in neurons and hepatic stellate cells."Molec.Brain Res.. 67・1. 74-81 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshiaki Minami: "Involvement of primary afferent C-fibers in touch-evoked pain (allodynia) induced by prostaglandin E2"Eur.J.Neurosci.. 11・6. 1849-1856 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masahiko kase: "Distribution of neurons expressing α1G subunit mRNA of T-type voltage-dependent calcium channel in adult rat central nervous system"Neurosci.Lett.. 268・2. 77-80 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Noriyuki Nishida: "A mouse prion protein (PrP) transgene rescues Purkinje cell degeneration and demyelination in mice deficient for PrP"Lab.Invest.. 79・6. 689-697 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osamu Minowa: "Altered cochlear fibrocytes in a mouse model of DFN3 nonsyndromic deafness"Science. 285 5432. 1408-1411 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Teizo Ueyama: "Comparative distribution of GABAergic and peptide-containing neurons in the lateral lemniscal nuclei of the rat."Brain Res.. 849・1,2. 220-225 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-03-31   Modified: 2016-04-21  

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