| Project/Area Number |
11554035
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
植物生理
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IKEUCHI Masahiko The University of Tokyo, Graduate School of Arts and Sciences, Associate Professor, 大学院・総合文化研究科, 助教授 (20159601)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIURA Miwa Osaka Prefecture University, Faculty of Agriculture, Research Assistant, 農学部, 助手 (80312255)
HIRANO Masahiko Toray Research Institute, Chief Scientist, 生物研究部, 次長(研究職)
JIAN-REN Shen RIKEN Harima Institute, Photosynthesis Research Laboratory, Senior Researcher, 光合成研究室, 研究員 (60261161)
井上 頼直 東京大学, 理化学研究所・光合成研究, 主任研究員 (70087592)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥10,200,000 (Direct Cost: ¥10,200,000)
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| Keywords | cyanobacteria / blue-green algae / photosynthetic production / thermophile / gene manipulation / gene expression / photosystem II / crystallization / シャトルベクター |
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| Research Abstract |
A transformation-competent clone of the thermophilic cyanobacterium Thermosynechococcus elongatus strain BP-1 was established. In collaboration with S. Tabata (Kazusa DNA Res. Inst.), we determined the complete nucleotide sequence.
A gene for a novel flavin-binding protein was cloned from T. elongatus and expressed in E. coli.
Phycocyanin was isolated from T. elongatus and crystalled. X-ray diffraction data at 2.0 A resolution was obtained.
Gene disruption mutants were created for psbI, psbT, psbV and psbK in T. elongatus. Photosystem II complexes were isolated from these mutants.
A number of site-directed mutants were constructed for the extrinsic 33 kDa protein of T. elongatus and were analyzed functionally.
O_2-evolving Photosystem II complex was isolated from wild type of T. vulcanus and crystallized. The 3D
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