| Project/Area Number |
11670913
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | KEIO University |
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Principal Investigator |
SHIGEMATSU Naoyuki School of Medicine, KEIO University Associate Professor, 医学部, 専任講師 (30178868)
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| Co-Investigator(Kenkyū-buntansha) |
OSAMU Kawaguchi School of Medicine, KEIO University Assistant, 医学部, 助手 (90276428)
ISHIBASHI Ryochi School of Medicine, KEIO University Assistant, 医学部, 助手 (90286584)
KUTSUKI Shouji School of Medicine, KEIO University Assistant, 医学部, 助手 (00234443)
KASAMATSU Tomotaka School of Medicine, KEIO University Assistant, 医学部, 助手 (30317242)
佐藤 導直 慶應義塾大学, 医学部, 助手 (80255554)
戸矢 和仁 慶應義塾大学, 医学部, 助手 (30265912)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Radiotherapy / Chromosomal aberration / Total body irradiation / Late-radiation toxicity / 放射線照射 / 細胞周期 / アポトーシス / 遺伝子突然変異 |
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| Research Abstract |
We have studied the prediction assay of radiation induced secondary malignancy by using in vitro human cell lines. Previously, we reported the comparison of the mutation induction (hprt locus) by heavy ion beams and X-rays when cells were irradiated. In this report, we described the chromosomal aberration (dicentric and fragment) of human lymphocyte taken from patients before and after total body irradiation (TBI). Bloods were drawn several times during the TBI and lymphocytes were isolated, and then observed by Giemsa-staining. We also took lymphocytes from patients before TBI, and then they were irradiated under the same schedule of TBI, and evaluated in * same assay. The dicentric frequencies and fragment numbers were well related with radiation dose both in vivo (TBI) and in vitro studies (figure1.2). Our experimental results indicated that this assay system would be applicable to lymphocytes from patients who receive irradiation therapy.
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