| Project/Area Number |
11671353
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Gunma University |
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Principal Investigator |
TAKAHASHI Akio Gunma University, School of Medicine, Assist. prof., 医学部, 助手 (60261856)
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| Co-Investigator(Kenkyū-buntansha) |
NEGISHI Masatoshi Gunma University, School of Medicine, Assist. prof., 医学部, 助手 (80272235)
KOBAYASHI Satoshi Gunma University, School of Medicine, Assist. prof., 医学部, 助手 (00265779)
FUJIMAKI Hiroya Gunma University, School of Medicine, Assist. prof., 医学部, 助手 (80292613)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | experimental rat pituitary adenoma / single high-dose radiotherapy / prolactinoma / 1回大線量照射 / prolactinoma / radiosurgery / diethylstilbestrol / rat |
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| Research Abstract |
Background : Single high-dose radiotherapy, i.e. gamma knife, is remarkable for the treatment for human prolactinoma. Objective and hypothesis : To clarify the hormonal and pathological effects of single high-dose radiotherapy for human prolactinoma, we used rat prolactinoma model. Method : A 5-week-old male Fischer 344 rat was subcutaneously implanted with 5 mg of diethylstilbestrol (DES) Seven weeks later, prolactinoma was induced and the tumor was treated with single high-dose radiotherapy with 10 mm collimator. Four weeks later, the rats were sacrificed, and weight of pituitary gland, serum prolactin(PRL) and Ki-67(MIB-5) labeling index were measured. Results : Of the control group (n=4), weight of pituitary gland was 7.2 mg, serum PRL was 35.8 ng/ml and MIB-5 labeling was 0.59 %. Of the rats with DES implant and without radiotherapy (n=5) weight of pituitary gland was 11.6 mg, serum PRL was 559 ng/ml and MIB-5 labeling was 0.88 %. Of the rats with DES implant and with 16 Gy radiotherapy (n=4), weight of pituitary gland was 11.1 mg, serum PRL was 121 ng/ml and MIB-5 labeling was 0.46 %. Of the rats with DES implant and with 32 Gy radiotherapy (n=5), weight of pituitary gland was 7.7 mg, serum PRL was 16.8 ng/ml and MIB-5 labeling was 0.38 %. Conclusions : Single high-dose radiotherapy is effective in the treatment for prolactinoma, dose-dependently. A dose of 32 Gy is recommended to practical use.
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