Project/Area Number |
11671622
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Obstetrics and gynecology
|
Research Institution | HIROSHIMA UNIVERSITY |
Principal Investigator |
NAGAI Nobutaka Hiroshima University, Faculty of medicine, associated professor, 医学部, 助教授 (90198292)
|
Co-Investigator(Kenkyū-buntansha) |
川上 洋介 広島大学, 医学部・附属病院, 医員
村上 順子 広島大学, 医学部, 助手 (80294560)
|
Project Period (FY) |
1999 – 2001
|
Project Status |
Completed (Fiscal Year 2001)
|
Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
Keywords | uterine cancer / ovarian cancer / telomerase / hTERT / GnRH / p16 / p27 / 婦人科癌 / hTERアンチセンス / 性ステロイドホルモン / アデノウィルスベクター / hTERCアンチセンス |
Research Abstract |
(1) Regulation mechanism of telomerase activity using cultured endometrial cells : The expression of telomerase activity was stimulated by endometrial stromal cells. The regulation controlled by sex steroid hormones has already finished at the early secretory phase in the menstrual cycle. (2) Expression of hTERT in endometrial cancer : Human TERT has regulated the telomerase activity and its detection is useful for the cancer diagnosis. (3) Relationship between p16 gene transfer and the telomerase activity. Adenovirus-mediated p16 gene transfer controlled the telomerase activity in ovarian cancer cell line OVCAR-5. (4) The RNA component of human telomerase antisense controlled the telomerase activity and cell proliferation. (5) GnRH agonist controlled cell proliferation and the expression of hTERT mRNA in endometrial cancer cell line. (6) Telomerase activity was lower in the endometrial cancer which had the expression of p27 gene compared with no expression of p27 gene.
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