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体液からの年齢推定に関する法医学的研究

Research Project

Project/Area Number 11897010
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section企画調査
Research Field Legal medicine
Research InstitutionGunma University

Principal Investigator

岸 紘一郎  群馬大学, 医学部, 教授 (30169841)

Co-Investigator(Kenkyū-buntansha) 安田 年博  群馬大学, 助教授 (80175645)
滝澤 久夫  富山医科薬科大学, 医学部, 教授 (90171579)
三澤 章吾  筑波大学, 社会医学系, 教授 (50086534)
飯田 礼子  福井医科大学, 医学部, 助手 (40139788)
竹下 治男  群馬大学, 講師 (90292599)
Project Period (FY) 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥3,300,000 (Direct Cost: ¥3,300,000)
Keywords個人識別 / 年齢推定 / 体液 / 分子生物学 / 尿
Research Abstract

法医学において個人識別は極めて重要な研究分野の一つであるが、現在のところ血液などの体液から年齢推定可能な年齢依存性変化を示すマーカーはなく、これらマーカの発見・法医学実務への導入が図られなければならない。そこで、本研究では血液や体液から年齢推定に活用しうるマーカーを開発すべく予備実験、文献調査および討議を重ね、本課題を将来「重点領域研究」の研究課題とする予備調査を目的とした。
1.従前の研究において17歳以下の若年者尿中には存在するが、それ以上になると検出できなくなる年齢依存性タンパク質(Ugl-Y)を見出した。今回、若年者尿から精製したUgl-Yに関するタンパク質化学的解析から、Ugl-Yの全アミノ酸配列の95%以上を決定した。さらに、その構造はfibronectinの一領域にほぼ一致することが明らかとなった。2.年齢推定に利用しうる年齢依存性生体分子を検索するため分子生物学的アプローチとして遺伝子発現パターンの年齢依存性変動を精査した。遺伝子発現の変動をモニターする有効な方法であるfluorescence differential display(FDD)-PCR法を利用して、異なった成長段階におけるマウス腎臓の遺伝子発現を解析した。その結果、7種類の遺伝子で有意な年齢依存的発現の認められることを見出した。そのなかには老年期のみに発現されるものなど様々な年齢依存的発現パターンが観察された。本研究結果はFDD-PCR法が年齢依存的発現を示す遺伝子の検索には有効な手段であることを証明するものであった。今後は、本法によって見出された年齢依存的発現を示す遺伝子について、その産物が法医学的試料から検出しうるものか否かを検討して年齢推定マーカーとして活用する予定である。
以上の研究成果、文献調査などから、年齢依存性マーカーの発見・実務への応用に関する基本的コンセンサスが得られ、それに沿って研究を継続する予定である。

Report

(1 results)
  • 1999 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Reiko Iida: "Deoxyribonuclease I phenotyping from saliva stains"The Journal of Forensic Sciences. 44(1). 175-178 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Structural requirement of human deoxyribonuclease II for the development of the active enzyme form,revealed by site-directed mutagenesis"Biochemocal and Biophysical Research Communications. 256(3). 591-594 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osamu Hosomi: "Xenopus laevis pancreatic Dnase I:purification and characterization"Experimental and Clinical Immunogenetics. 19(2). 117-123 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "A new allele,DNASE1^*6,of human deoxyribouuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability"Biochemical and Biophysical Research Communications. 260(1). 280-283 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Haruo Takeshita: "Simultaneous typing of the STR loci,vWF and HumTPO,using discontinuous polyacrylamide gel electrophoresis"Legal Medicine. 1(3). 233-237 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshimitsu Nakashima: "Molecular,biochemical and immunological studies of hen pancreatic deoxyribonuclease I"International Journal of Biochemistry and Cell Biology. 31(11). 1313-1324 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Einosuke Tanaka: "Clinically significant pharmacokinetic drug interactions with psychoactive drugs"Journal of Clinical Pharmacy and Therapeutics. 24. 7-16 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuya Honda: "Identification of a dismembered body by DNA analysis"International Medical Journal. 61(2). 151-154 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshihiko Kominato: "Expression of human histo-blood group ABO genes is dependent upon DNA methylation of the promoter region"Journal of Biological Chemistry. 274. 37240-37250 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Reiko Iida: "Age-related changes of gene expression in mouse kidney:fluorescence differential display-PCR analysis"Mechanisms of Ageing and Development. 113(2). 133-142 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshihiko Kominat: "Acute cerebellar hemorrhage in a patinet with Klinefelter syndrome"The Journal of Forensic Sciences. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Ichiro Shimada: "DNA polymorphisms in the 5'-flanking sequence of human ABO blood group genes and their association with the alleles for the common ABO phenotypes"Legal Medicine. 1(4). 217-225 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akira Kido: "AmpliType PM+DQA1 typing on aged seminal stains"Acta Criminologiae et Medicinae Legalis Japonica. 65(2). 66-69 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] I.Kondo: "Missense mutations in phosphomannomutase 2 gene in two Japanese families with carbohyrate-deficient glycoprotein syndrome type 1"Clinical Genetics. 55(1). 50-54 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Zang: "Novel TSC2 mutation in patient with pulmonary tuberous sclerosis:lack of loss of heterozygosity in a lung cyst"American Journal of Medical Genetics. 82(5). 368-370 (1999)

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      1999 Annual Research Report
  • [Publications] M.Nakagawa: "A new single-nucleotide polymorphism in the seventh componenet of complement (C7) gene"Journal of Human Genetics. 44(4). 272-273 (1999)

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      1999 Annual Research Report
  • [Publications] K.Mizugishi: "Missenes mutations in the phosphomannomutase 2 gene of two Japanese siblings with carbohydrate-deficient glycoprotein syndrom type 1"Braon and Development. 21(3). 223-228 (1999)

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      1999 Annual Research Report
  • [Publications] K.Yoshikawa: "Determination of carbohydrate-deticient transterrin separated by lectin affinity chromatography for detecting chronic alcohol abuse"FEBS Letters. 458(2). 112-116 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tamiko Nakajima: "Rapid purification of human Dnase I using mouse monoclonal anti-Dnase I antibodies and characterization of the antibodies"Experimental and Clinical Immunogenetics. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiro Yasuda: "The molecular basis for genetic polymorphism of human deoxyribonuclease II (Dnase II)"FEBS Letters. 467(1/2). 231-234 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Osamu Hosomi: "Molecular cloning of cDNA encoding Xenopus Laevis deoxyribonuclease I"DNA Sequence. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] 藤川克行: "日本人のトランスフェリン多型に認められる地理的勾配(埼玉県および青森県在住者を中心として)"血液事業. 21(4). 279-287 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 安田年博: "ヒトdeoxyribonuclease II(Dnase II)に関する最近の研究"北関東医学. 49(1). 39-41 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 岸紘一郎: "Dnase I"日本臨床. 57(増刊). 486-489 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 竹下治男: "不連続系ポリアクリルアミドゲル電気泳動を利用した2種類のSTR座位(vWFおよびHumTPO)の同時検出"DNA多型. 7. 88-90 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 渡邊剛太郎: "Heteroduplex analysis によるSTR多型解析"DNA多型. 7. 124-128 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 三次綾: "西日本と韓国におけるalchoholdehydrogenase 2(ADH2)およびaldehyde dehydrogenase 2(ALDH2)の遺伝的多型について"DNA多型. 7. 158-160 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 梅津和夫: "凝固因子FXIIIBとSTR多型と蛋白多型との関連について"DNA多型. 7. 241-243 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 村脇義和: "ALDH2遺伝子型の飲酒習慣およびアルコール性肝障害への影響:健康診断受診者での検討"アルコールと医学生物学. 19. 74-77 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 三澤章吾: "自動車事故における人体損傷の形成機序"法医学の実際と研究. 42. 1-16 (1999)

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      1999 Annual Research Report
  • [Publications] 三澤章吾: "ミクロからマクロへ―個から集団へ"學鐙. 96(3). 10-13 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Haruo Takeshita: "Postmortem absorption of dichloromethane:a case study and animal experiments"International Journal of Legal Medicine. (in press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Haruo Takeshita: "Mammalian deoxyribonucleases I are classified into three types:pancreas,parotid and pancreas-parotid(mixed) types,based on differences of their tissue concentrations"Biochemical and Biophysical Reseach Communications. (in press).

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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