| Project/Area Number |
12306009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
ITOH Takao Wood Research Institute, Professor, 木質科学研究所, 教授 (70027168)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Kei'ichi Wood Research Institute, Lecturer, 木質科学研究所, 助手 (20238223)
SUGIYAMA Junji Wood Research Institute, Associate Professor, 木質科学研究所, 助教授 (40183842)
木村 聡 京都大学, 木質科学研究所, 非常勤研究員
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| Project Period (FY) |
2000 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥33,990,000 (Direct Cost: ¥31,500,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2002: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2001: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2000: ¥23,200,000 (Direct Cost: ¥23,200,000)
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| Keywords | Cellulose synthases / GFP / GhCesA::GFP proteins / セルロース / セルロース生合成 / TCの移動 / ロゼット / 蛍光標識 / ターミナルコンプレックス / レーザー顕微鏡 |
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| Research Abstract |
(1) It was demonstrated that part of cellulose synthase, c-di-GMP binding domain, is localized in the specific particle rows in cell membrane.
(2) GhCesA::GFP was prepared for the visualization of TC movement
(3) Three different techniques that is microinjection, electro-polation, and particle-gun was examined to introduce GhCesA::GFP proteins into tobacco BY2 cells. The particle-gun method was the best to introduce the proteins in the cells with a little damages.
(4) The network structure was confirmed as endoplasmic reticulum (ER) by comparing the fluorescence pattern of GFP-fused proteins and DiOC6 staining images specific to ER.
(5) It was shown that the partial CesA3 gene can be expressed in ER.
(6) It was suggested that ChCesA::GFP proteins were localized in Golgi bodies as well by the distribution pattern of sphere-like fluorescence and their diameter similar to Golgi bodies.
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