MATSUDA Yoichi Hokkaido University, Center for Advanced Science and Technology, Professor, 先端科学技術共同研究センター, 教授 (70165835)
NISHIBORI Masahide Hiroshima University, Graduate School of Biosphere Science, Assistant Professor, 大学院・生物圏科学研究科, 助手 (80237718)
NISHIMURA Toshihide Hiroshima University, Graduate School of Biosphere Science, Professor, 大学院・生物圏科学研究科, 教授 (70180643)
TAKAHASHI Hideaki National Institute of Agrobiological Sciences, Chief Researcher, 主任研究員
|Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 2002: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2001: ¥3,500,000 (Direct Cost: ¥3,500,000)
Based on Oh-Shamo and White Leghorn breeds of chickens, an F_2 resource family was established for quantitative trait loci (QTL) analysis. Microsatellite DNA was used as a marker. As a result of a QTL analysis, a highly significant (LOD score = 22.2) QTL was found on Chromosome 1, with regard to the shank length. Another QTL analysis revealed that there are several significant QTLs (LODscore > 4.4) on Chromosomes 1, 2, 3, 5, 7, 12, and 28 concerning egg weight at 300 days of hen age. Furthermore, with regard to egg production rate at 300 and 400 days of hen age, significant QTLs were discovered on Chromosomes 1, 2, 3, 4, 7, 9, and 28, and on Chromosomes 1, 3, 4, 7, 9, and 28, respectively.
In addition to the QTL analyses mentioned above, 600 microsatellite markers were newly developed and mapped to 27 linkage groups. The genetic length of the map was 2,600 cM, and the average length between respective markers was 9.8 cM. This is the first genetic linkage map in Japan that was made based on domestic microsatellite markers.