|Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
We showed that SHP-2, a tyrosine phosphatase, plays an important role in cell locomotion, using dominant negative mutant.(Oncogene, 19:75-84,2000). We also found that Dok-1, a docking protein, plays an important role in the movement of B-16 melanoma cells (Mol Cell Biol, 21:5437-5446, 2001). We showed that IL-18 synergistically induces tumor rejection with IL-12 which is locally secreted by IL-12 transfected B-16 melanoma cells in vivo (Cancer Invest, 18;206-13, 2000). Using IL-12 transfected melanoma cells, we showed that CD4+T cell depletion induces both melanoma rejection and vitiligo-like depigmentation (J Invest Dermatol, 115:1059-1064,2000). In this regards, we found that IL-12 restores UV-induced suppression of resposiveness of Tlymphocytes (J Derm Sci, 24:190-202,2000). We also reported that giant melanosomes in Hermansky Pudlak syndrome which has a freature of impaired transportation of melanogenic enzymes in pigment cells (Br J Dermatol, 143:635-640,2000). Further, we showed that rab3A, an important G protein for intracellutar vgesicular transportation, is associated with melanosomes in pigment cells (Pigment Cell Res, 13:332-336,2000). Our findings will provide a useful information in establishing a new strategy of the treatment for depigmented disorders.