| Project/Area Number |
13470442
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
TSUCHIMOCHI Makoto The Nippon Dental Univ., school of dentistry Niigata, Professor, 新潟歯学部, 教授 (20095186)
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| Co-Investigator(Kenkyū-buntansha) |
HARADA Mikiko The Nippon Dental Univ., school of dentistry Niigata, Lecturer, 新潟歯学部, 助手 (60339471)
KAWASE Tomoyuki Niigata University, Graduate School Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (90191999)
SAITO Eiichi The Nippon Dental Univ., school of dentistry Niigata, Associate Professor, 新潟歯学部, 助教授 (40120662)
KAMETA Ayako The Nippon Dental Univ., school of dentistry Niigata, Lecturer, 新潟歯学部, 助手 (00328866)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2002: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥9,200,000 (Direct Cost: ¥9,200,000)
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| Keywords | PTHrP / cDNA Array / squamous cell carcinoma / oral cancer / differentiation / keratinocyte / cell cycle / hTERT / PTHrP遺伝子 / ケラチノサイト / ケラチン |
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| Research Abstract |
In previous clinical immunohistochemical studies, the authors found that parathyroid hormone-related protein (PTHrP) was expressed in oral squamous cell carcinoma in which the localization corresponded to regions of higher keratinization and tower grade of histologic malignancy. The purpose of this study was to examine the effect of PTHrP on proliferation and differentiation of oral squamous cells. We used a squamous carcinoma cell line, SCC-25, derived from tongue cancer, and an immortalized keratino cyte cell line, NDUSD-1, derived from human gingival tissue. PTHrP (1-34), (34-53), and (107-139) fragments were added to the culture medium. It has been reported that each of PTHrP fragments has different physiologic functions, such as regulation of smooth muscle tone, transepithelial calcium transport, and tissue and organ development, differentiation, and proliferation. For assessment of proliferation, we counted the number of cells. The ability of the fragments to affect differentiation was evaluated by using immunofluorescent staining with involucrin, cytokeratin (CK)10, CK14, CK5, CK6, and CK18. We also assessed intracellular cAMP concentration. No changes in cell number or immunofluorescence staining were evident in either cell line following stimulation with the fragments. The concentration of cAMP did not change in either cell line after adding the fragments. PTHrP fragment (1-34) did not increase CK expressions in NDUSD-1 in a cDNA microarray study. Although it has been reported that PTHrP affects differentiation of keratinocytes on antisense RNA study, we did not observe any influence of exogenously added PTHrP on cell differentiation and proliferation in the examined cell lines. The endogenous function of the fragments in oral squamous cells requires further study. Furthermore we examined in vitro effects of PTHrP by using cDNA array study. PTHrP could not reduce gene expression affecting cell proliferation.
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