| Project/Area Number |
13670031
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Kansai Medical University |
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Principal Investigator |
WATANABE Jun Kansai Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (40148557)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | arylhydrocarbon receptor / HSP90 / transcriptional regulation / CYP1A1 / CYP2B2 / Neural tissues / liver / rat / arylhydrocarbon receptor / 細胞内輸送 / メチルコランスレン / 脳 |
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| Research Abstract |
1.By the chemiluminescent or fluorescent immunohistochemistry, HSP90 localized with arylhydrocarbon receptor(AhR) was dissociated from AhR in the cytoplasm but not in the nuclei after the stimulation of 3 -methylcholanthrene in the H4IIE cell lines and hepatocytes in sections from rat livers. However, FLET analysis revealed that the both proteins associated with a loose connection or with interconnecting factor(s). We applied this technique to examine the intracellular localization of a novel protein that localized exclusively in the endocrine cells.
2.Aclose relationship was found between the expression of CYP2B2 and area of endoplasmic reticulum(ER) in hepatocytes from normal and phenobarbital-treated rats as revealed by a combination of highly-sensitive and quantitative in situ hybridization of CYP2B2 mRNA with stereological analysis of ER.
3.When a reporter gene was connected to 5'-region of CYP1A1 gene, we could detect the localization of the gene by the photon imaging. However, no significant difference was found in the DNA structure if 5 '-region of CYP1A1 gene and AhR-Arnt were reacted under the presence of transcriptional factors, Sp1 or CBP/p-300 by the photon detection and/or atomic-force microscopy.
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