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Inhibitory effect of human α_2-plasmin inhibitor and it's intensified compound by S-nitrosylation on the activity of arginine-specific cysteine proteinase from Porphyromonas gingivalis.

Research Project

Project/Area Number 13671931
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionFukuoka Dental College

Principal Investigator

KAMINISHI Hidenori  Fukuoka Dental College, Infection Biology, Professor, 歯学部, 教授 (90084300)

Co-Investigator(Kenkyū-buntansha) AKAIKE Takaaki  Kumamoto University, Medical Microbiology, Professor, 医学部, 助教授 (20231798)
TAKEUCHI Hisako  Fukuoka Dental College, Infection Biology, Research Assistant, 歯学部, 助手 (50198800)
OGURA Rieko  Fukuoka Dental College, Infection Biology, Research Assistant, 歯学部, 助手 (80320331)
HAMAMOTO Takayoshi  The Chemo-Sero-Therapeutic Research Institute, Research Fellow, 研究員
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2001: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsP. gingivalis / gingipain / α_2-plasmin inhibitor / S-nitrosylation / α2アンチプラスミン / α1プロテアーゼインヒビター / ベジクル / vesicle / α-2アンチプラスミン / Porphyromonas gingivalis
Research Abstract

We investigated the effect of human α_2-plasmin inhibitor (α_2PI) and S-nitrosylated α_2-plasmin inhibitor (S-NO-α_2PI) on the activity of arginine-specific cysteine proteinase (gingipain, RGP) from Porphyromonas gingivalis, an aetiological bacterium of adult periodontitis, with the use of fluorogenic substrates. We discovered that human α_2PI as well as S-NO-α_2PI play a inhibitory effect to the RGP in a dose-dependent manner. The α_2PI, one of the members of serpin family, is well known as a specific inhibitor of plasmin, a typical serine proteinase. However, few studies have been reported concerning the inhibitory potency of humoral factors, such as α_1-protease inhibitor or α_2-macroglobulin, against cysteine proteinases especially RGP. In this study, we found a novel function of α_2PI as a potent inhibitor of RGP. The inhibitory potencies of the S-nitrosylated derivative against both human plasmin and RGP were slightly higher than those of the intact α_2PI. These results strongly suggest that S-NO-α_2PI is a useful tool for preventing the virulence of P. gingivalis.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 上西秀則, 小倉理恵子 ほか: "α_2プラスミンインヒビターおよびそのニトロソ化物はPorphyromonas gingivalisプロテアーゼの活性を阻害する"医学と生物学. 148. 27-31 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 上西秀則, 長 環, 小倉理恵子: "真菌の遺伝学"日本臨牀. 61. 785-792 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inoue Y, Fukushima T., et al.: "Antibacterial characteristics of newly developed amphiphilic lipids and DNA-lipid complexes against bacteria."J.Biomed.Mater.Res.. 65A. 203-208 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cho T., Toyoda M., et al.: "Isolation and sequencing of the Candida albicans MSI3, a putative novel member of the HSP7O family."Yeast. 20. 149-156 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hidenori KAMINISHI, Rieko OGURA, Mika TOYODA, Tamaki CHO, Takayoshi HAMAMOTO: "Inhibitory Effect of Human α_2-Plasmin Inhibitor and It's S-Nitrosylated Derivative on the Activity of Arginine-Specific Cysteine Proteinase from Porphyromonas gingivalis."Medicine and Biology. 148,(6). 27-31 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 上西秀則, 小倉理恵子 ほか: "ニトロソ化によるα2プラスミンインヒビターの活性増強"医学と生物学. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 上西秀則, 長 環, 小倉理恵子: "真菌の遺伝学"日本臨牀. 61. 785-792 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inoue Y., Fukushima T., et al.: "Antibacterial characteristics of newly developed amphiphilic lipids and DNA-lipid complexes against bacteria."J.Biomed.Mater.Res.. 65A. 203-208 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Cho T., Tpyoda M., et al.: "Isolation and sequencing of the Candida albicans MSI3, a putative novel member of the HSP70 family."Yeast. 20. 149-156 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Inoue, Y., Fukushima, T., et al.: "Antibacterial Characteristics of Newly Developed Amphiphilic Lipids and DNA-lipid Complexes against Bacteria"Journal of Biomedical Material Research. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Cho, T., M.Sudoh, H, Kaminishi et al.: "Isolation and sequencing of the Candida albicans MS13, a putative novel member of the HSP70 family"Yeast. 20. 149-156 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 上西秀則 ほか: "真菌の遺伝学"日本臨牀. 61・3. 785-792 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] T.Cho et al.: "Isolation and expression of a gene(CGR1) regulated during the yeast-hyphal transition in Candida albicans"Biochimica et Biophysica Acta. 1517巻. 288-292 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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