Molecular biological study on functional non-cariogenic tri-saccharide metabolized by microorganisms in the intestines
| Project/Area Number |
13672137
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
矯正・小児・社会系歯学
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| Research Institution | Tohoku University |
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Principal Investigator |
HATA Shinji TOHOKU UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, DEPARTMENT OF ORAL HEALTH SCIENCES DEVISION OF PEDIATRIC DENTISTRY, LECTURER, 大学院・歯学研究科, 講師 (20208507)
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| Co-Investigator(Kenkyū-buntansha) |
HATA Hiroko TOHOKU UNIVERSITY, GRADUATE SCHOOL OF DENTISTRY, DEPARTMENT OF ORAL HEALTH SCIENCES, DIVISION OF PEDIATRIC DENTISTRY, ASSISTANT RESEARCHER, 大学院・歯学研究科, 助手 (20180630)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | LACTOSUCROSE / ORAL BACTERIA / ACTINOMYSES |
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| Research Abstract |
1.Growth : A. odontolyticus ATCC 17982 utilized galactose a little. A. odontolyticus ATCC 17982 utilized LF more than galactose, but not as much as glucose, sucrose, lactose and fructose. S.oralis ATCC 10557 utilized all carbohydrate used in this study, but the rate of utilization of galactose and LF were slower than other sugars used in this study.
2.Degradation pattern : Galactose was detected and accumulated in the supernatant of the medium while trace amount of the other mono-and di-saccharides were detected when A.odontolyticus ATCC 17982 and S.oralis ATCC 10557 had been grown in TTY-LF. Galactose had remained in the supernatant of the medium of TTY-Glac-Gal-Fru and TTY-Suc-Lac. S.oralis ATCC 10557 degraded the sugars more rapidly than A.odontolyticus ATCC 17982.
3.β-Galactosidase : The maximum activities of β-galactosidase of A.odontolyticus ATCC 17982 was 0.22 μmol/min/mg, which was detected in 3 hours after addition of LF although those of S.oralis ATCC 10557 was 0.486 μmol/min/mg, which was detected whthin 30 min.
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Report
(4 results)
Research Products
(2 results)
