| Project/Area Number |
14350495
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
高分子構造・物性(含繊維)
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| Research Institution | Shinshu University |
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Principal Investigator |
ABE Koji Shinshu University, Faculty of Textile Science and Technology, Professor, 繊維学部, 教授 (00126658)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Toshihiro Shinshu University, Faculty of Textile Science and Technology, Professor, 繊維学部, 教授 (30126700)
FUJII Toshihiro Shinshu University, Faculty of Textile Science and Technology, Professor, 繊維学部, 教授 (50126702)
SASAKI Katsunori Shinshu University, School of Medicine, Professor, 医学部, 教授 (30170666)
MATSUDA Naoki Nagasaki University, Center for Frontier Life Sciences, Division of Radiation Biology and Protection, Professor, 先導生命科学研究支援センター・アイソトープリソース開発分野, 教授 (00304973)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2004: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2002: ¥8,600,000 (Direct Cost: ¥8,600,000)
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| Keywords | Regenerative Medicine / Artificial Organ / Biofiber / Extracellular Matrix / Cartilage Cell / Polysaccharide / Polyelectrolyte Complex / Osteoblast / 人工臓器 / 胚性幹細胞 / ストレス応答 / 細胞内シグナル伝達 |
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| Research Abstract |
In this investigation, we have examined the control of cell specific functions (proliferation and differentiation) of the hard tissue cells (bone and chondrocyte) by polyelectrolyte complex (PEC), which are composed of polysaccharides.
Osteoblasts cultured on PEC containing sulfated polysaccharide (SPEC) were proliferated similar to tissue culture dish (TCD) and exhibited spreading form. On the other hand, the proliferation of cell cultured on PEG containing carboxymethylated polysaccharide (OPEC) coated dishes was slower than those on TCD and exhibited aggregate form. Expression of ALPase activity and deposition of calcium, which were initial differentiation markers of calcification, were observed mainly at the part of cell aggregate. Further, expression of mRNA of osteoblastic differentiation markers (osteocalcin and osteopontin) were observed on OPEC earlier than on SPEC and TCD. N-cadherin, cell-cell adhesion protein, expression was strong and localized at the cell-cell interfaces in the cell aggregates.
Chondrocyte (ATDC5) cultured on SPEC grew well in the similar manner to TCD with spreading form. On the other hand, the growth of ATDC5 on OPEC was remarkably suppressed and exhibited cell aggregate form. The sizes of these aggregates were made bigger with time. These phenomena are well coincident with the culture of osteoblasts on these PECs. Secretion of chondrocyte matrix and expression of mRNA of typeII collagen were promoted on the OPEC.
These results suggested that the cell functions, proliferation and differentiation, are mainly controlled by the chemical structures of anionic polysaccharides ; the existence of carboxylic acid residues induces the differentiation and sulfonic acid residues promotes the proliferation of cells.
In conclusion, polysaccharides-type PEC are excellent biomaterials for hard tissue cell culture.
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