| Project/Area Number |
14571851
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Kyushu University |
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Principal Investigator |
SAKAI Takako Kyushu University, University Hospital, Assistant Professor, 病院, 講師 (60128022)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUO Kou Kyushu University, Faculty of Dental Science, Associate Professor, 大学院・歯学研究院, 助教授 (70238971)
KOBAYASHI Ieyoshi Kyushu University, Faculty of Dental Science, Assistant Professor, 大学院・歯学研究院, 講師 (40243951)
SAKAI Hidetaka Kyushu University, Faculty of Dental Science, Professor, 大学院・歯学研究院, 教授 (80136499)
TERADA Yoshihiro Kyushu University, Faculty of Dental Science, Professor, 大学院・歯学研究院, 教授 (30038898)
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| Project Period (FY) |
2002 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | age-dependent change / gingival tissue / apoptosis / BrdU / TUNEL / SMP-30c / DNA subtraction / 加齢 / 老化遺伝子 |
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| Research Abstract |
We previously examined the age-dependent change of BrdU- and TUNEL-positive cells in the mouse periodontal tissue, and showed that a significant decrease of cellular component in association with aging occurred in the submucosal connective tissue caused by apoptosis. Based on this result, we also examined the cell kinetic change of various organs and tissues in mouse with aging. Decrease of BrdU-positive cells was observed in the epithelial layer of gingiva, buccal mucosa, skin and hair follicle at 30 weeks and following ages after birth. Meanwhile, increase of TUNEL-positive cell was demonstrated gingival submucosal tissue, and mucinous and serous acinar cells of submandibular gland at 20 weeks and following ages after birth. Thus age dependent change of the cell kinetic is evident in the gingival tissue, and is therefore seemed to be related with gingival recession.
Senecent marker protein-30(SMP-30) is age-related marker protein, and is known to be expressed in brain and liver. SMP-30 is known to be related the apoptosis-resistant activity in cells. We examined the expression of SMP-30 in the mouse gingival tissue at 15 weeks after birth by RT-PCR. Furthernmore, we performed cDNA subtraction between 10 and 15 weeks aged mouse gingival tissue, and found that 68 genes in the 10 weeks, and 51 genes in the 15 weeks, were differentially expressed. Molecular interaction of SMP-30 and these differentially expressed genes are now proceeding.
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