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Mechanizms of Regionalizaion of the tectum and cerebellum and retinotectal projection

Research Project

Project/Area Number 15207016
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTohoku University

Principal Investigator

NAKAMURA Harukazu  Tohoku University, Department of Molecular Neurobiology, Graduate School of Life Sciences, Professor, 大学院・生命科学研究科, 教授 (90079690)

Co-Investigator(Kenkyū-buntansha) FUNAHASHI Junichi  Tohoku University, Department of Molecular Neurobiology, Institute of Development, Aging & Cancer, Associate Professor, 加齢医学研究科, 助教授 (00270827)
WATANABE Yuji  Tohoku University, Department of Molecular Neurobiology, Graduate School of Life Sciences, Assistant professor, 大学院・生命科学研究科, 助手 (80301042)
片平 立矢  東北大学, 大学院・生命科学研究科, 日本学術振興会特別研究員(PD)
佐藤 達也  東北大学, 大学院・生命科学研究科, 日本学術振興会特別研究員
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥50,310,000 (Direct Cost: ¥38,700,000、Indirect Cost: ¥11,610,000)
Fiscal Year 2005: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2004: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2003: ¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
Keywordschick embryo / cerebellum / Fgf8-Ras-ERK signaling cascade / organizer / Sprouty2 / regulation / misexpression / tectum / 峡部 / Fgf8 / Ras-ERKシグナル伝達系 / エレクトロポレーション / Ras-ERKシグナル経路 / siRNA / 遺伝子発現阻害
Research Abstract

The isthmus works as an organizer, and Fgf8 mimics the organizing activity. The isthmic organizer plays a role for the development of the tectum and the cerebellum in the mesencephalon and in the metencephalon, respectively. It was previously shown that strong Fgf8 signal induces cerebellar differentiation. In the present study, we have shown that strong Fgf8 signal activates Ras-ERK signaling cascade. If we shut down this signaling cascade by misexpression of dominant negative form of Ras (DN-Ras), y electroporation the fate of the metencephalon changes to the mesencephalon, and the tectum differentiates in place of the cerebellum. Then, we focused on Sprouty2. Sprouty2 is expressed overlapping to Fgf8, but was indicated as a negative regulator of Ras-ERK signaling cascade. As indicated, Sprouty2 expression was induced rapidly by Fgf8, that is, 2 hours after electroporation of Fgf8 expression vector, and repressed ERK activation. Misexpression of Sprouty2 exerted similar results as that of DN-Ras, that is, fate change of the metencephalon to the mesencephalon. On the other hand, misexpression of dominant negative form of Sprouty2 resulted in anterior shift of the mes-metencephalic boundary. The results indicate the need of fine regulation of Ras-ERK signaling.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (41 results)

All 2006 2005 2004 2003 Other

All Journal Article (34 results) Publications (7 results)

  • [Journal Article] Functional Analysis of Chick ONT1 Reveals Distinguishable Activities among Olfactomedin-Related Signaling Factors.2006

    • Author(s)
      Sakuragi, M., Sasai, N., Ikeya, M., Kawada, M., Katahira, T., Nakamura, H., Sasai,Y.
    • Journal Title

      Mech Develop. 123

      Pages: 114-123

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional Analysis of Chick ONT1 Reveals Distinguishable Activities among Olfactomedin-Related Signaling Factors.2006

    • Author(s)
      Sakuragi, M., Sasai, N., Ikeya, M., Kawada, M., Katahira, T., Nakamura, H., Sasai, Y.
    • Journal Title

      Mech Develop.

      Pages: 114-123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional analysis of chick ONT1 reveals distinguishable activities among olfactomedin-related signaling factors2006

    • Author(s)
      Sakuragi M., Sasai N., Ikeya M., Kawada M., Katahira T., Nakamura H., Sasai Y.
    • Journal Title

      Mech Develop 123

      Pages: 113-123

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isthmus organizer and regionalization of the mesencephalon and metencephalon.2005

    • Author(s)
      Nakamura, H., Watanabe, Y.
    • Journal Title

      Int. J. Dev. Biol., 49

      Pages: 231-235

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of isthmic Fgf8 signal by Sprouty2.2005

    • Author(s)
      Suzuki-Hirano, A., Sato, T., Nakamura, H.
    • Journal Title

      Development, 132

      Pages: 257-265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of protogenin, a novel immunoglobulin superfamily gene expressed during early chick embryogenesis.2005

    • Author(s)
      Toyoda, R., Nakamura, H., Watanabe, Y.
    • Journal Title

      Gene Exp. Pattern, 5

      Pages: 778-785

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isthmus organizer and regionalization of the mesencephalon and metencephalon.2005

    • Author(s)
      Nakamura, H., Watanabe, Y.
    • Journal Title

      Int.J.Dev.Biol. 49

      Pages: 231-235

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of isthmic Fgf8 signal by Sprouty2.2005

    • Author(s)
      Suzuki-Hirano, A., Sato, T., Nakamura, H.
    • Journal Title

      Development 132

      Pages: 257-265

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of protogenin, a novel immunoglobulin superfamily gene expressed during early chick embryogenesis.2005

    • Author(s)
      Toyoda, R., Nakamura, H., Watanabe, Y.
    • Journal Title

      Gene Exp.Pattern 5

      Pages: 778-785

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isthmus organizer for midbrain and hindbrain development.2005

    • Author(s)
      Nakamura, H., Katahira, T., Matsunaga, E., Sato, T.
    • Journal Title

      Brain Res.Rev. 49

      Pages: 120-126

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Regulation of isthmic Fgf8 signal by Sprouty22005

    • Author(s)
      Suzuki-Hirano A., Sato T., Nakamura H.
    • Journal Title

      Development 132

      Pages: 257-265

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isthmus organizer for midbrain and hindbrain development.2005

    • Author(s)
      Nakamura H., Katahira T., Matsunaga E., Sato T.
    • Journal Title

      Brain Res.Rev. 49

      Pages: 120-126

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 峡部オーガナイザーによる小脳分化の制御2005

    • Author(s)
      仲村春和
    • Journal Title

      実験医学 23

      Pages: 127-132

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 小脳分化を誘導するFGFシグナルの調節機構2005

    • Author(s)
      鈴木-平野明日香, 仲村春和
    • Journal Title

      細胞工学 24

      Pages: 1046-1050

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 小脳の発生2005

    • Author(s)
      仲村春和
    • Journal Title

      Clinical Neuroscience 23

      Pages: 1353-1355

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Regulation of isthmic Fgf8 signal by Sprouty2.2005

    • Author(s)
      Suzuki-Hirano, A., Sato, Nakamura, H.
    • Journal Title

      Development 132

      Pages: 257-265

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Navigation of Trochlear axons along the midbrain-hindbrain boundary to neuropilin-22004

    • Author(s)
      Watanabe, Y., Toyoda, R., Nakamura, H.
    • Journal Title

      Development 131

      Pages: 681-692

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Possible role of Hes5 for the rostrocaudal polarity formation of the tectum.2004

    • Author(s)
      Kimura, J., Katahira, T., Araki, I., Nakamura, H.
    • Journal Title

      Dev. Growth Differ., 46

      Pages: 219-227

    • NAID

      10013279385

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Fgf8 signal causes cerebellar differentiation by activating Ras-ERK signaling pathway2004

    • Author(s)
      Sato, R., Nakamuram, H
    • Journal Title

      Development 131

      Pages: 4275-4285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Polarity and laminar formation of the optic tectum in relation to retinal projection2004

    • Author(s)
      Nakamura, H., Sugiyama, S.
    • Journal Title

      J. Neurobiol., 59

      Pages: 48-56

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gain- and loss-of-function in chick embryos by electroporation2004

    • Author(s)
      Nakamural, H., Katahira, T., Watanabe, Y., Funahashi,J
    • Journal Title

      Mech. Develop. 121,

      Pages: 1137-1143

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] How does Fgf signaling from the isthmic organizer induce midbrain and cerebellum development?2004

    • Author(s)
      Sato T, Joyner A.L, Nakamura H.
    • Journal Title

      Dev. Growth Differ. 46

      Pages: 487-494

    • NAID

      10014094184

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Navigation of Trochlear axons along the midbrain-hindbrain boundary to neuropilin-2.2004

    • Author(s)
      Watanabe, Y., Toyoda, R., Nakamura, H.
    • Journal Title

      Development 131

      Pages: 682-692

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Possible role of Hes5 for the rostrocaudal polarity formation of the tectum.2004

    • Author(s)
      Kimura, J., Katahira, T., Araki, I., Nakamura, H.
    • Journal Title

      Dev.Growth Differ. 46

      Pages: 219-227

    • NAID

      10013279385

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] The Fgf8 signal causes cerebellar differentiation by activating Ras-ERK signaling pathway.2004

    • Author(s)
      Sato, R., Nakamuram, H.
    • Journal Title

      Development 131

      Pages: 4275-4285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Polarity and laminar formation of the optic tectum in relation to retinal projection.2004

    • Author(s)
      Nakamura, H., Sugiyama, S.
    • Journal Title

      J.Neurobiol. 59

      Pages: 48-56

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Gain- and loss- of-function in chick embryos by electroporation.2004

    • Author(s)
      Nakamural, H., Katahira, T., Watanabe, Y., Funahashi, J.
    • Journal Title

      Mech Develop. 121

      Pages: 1137-1143

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] How does Fgf signaling from the isthmic organizer induce midbrain and cerebellum development?2004

    • Author(s)
      Sato T, Joyner AL, Nakamura H.
    • Journal Title

      Dev.Growth Differ. 46

      Pages: 487-494

    • NAID

      10014094184

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The Fgf8 signal causes cerebellar differentiation by activating Ras-ERK signaling pathway.2004

    • Author(s)
      T.Sato, H.Nakamura
    • Journal Title

      Development 131

      Pages: 4275-4285

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Gain- and loss-of-function in chick embryos by electroporation.2004

    • Author(s)
      Nakamura, H., Katahira, T., Watanabe, Y., Funahashi, J.
    • Journal Title

      Mech.Devel. 121

      Pages: 1137-1143

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The role of Grg4 in tectal laminar formation2003

    • Author(s)
      Sugiyama, S., Nakamura, H.
    • Journal Title

      Development 130

      Pages: 451-462

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene silencing in chick embryos with vector-based small interfering RNA system2003

    • Author(s)
      Katahira, T., Nakamura, H.
    • Journal Title

      Dev Growth Differ 45

      Pages: 361-367

    • NAID

      10011769024

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The role of Grg4 in tectal laminar formation.2003

    • Author(s)
      Sugiyama, S., Nakamura, H.
    • Journal Title

      Development 130

      Pages: 451-462

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene silencing in chick embryos with vector-based small interfering RNA system.2003

    • Author(s)
      Katahira, T., Nakamura, H.
    • Journal Title

      Dev.Growth Differ. 45

      Pages: 361-367

    • NAID

      10011769024

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Katahira, T., Nakamura, H.: "Gene silencing in chick embryos with vector-based small interfering RNA system"Develop.Growth Differ.. 45・3. 361-367 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Watanabe, Y., Toyoda, R., Nakamura, H.: "Navigation of Trochlear axons along the midbrain-hindbrain boundary to neuropilin-2"Development. 131・3. 681-692 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakamura, H., Sugiyama, S.: "Polarity and laminar formation of the optic tectum in relation to retinal projection"J.Neurobiol.. 59・1. 48-56 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kimura, J., Katahira, T., Araki, I., Nakamura, H.: "Possible role of Hes5 for the rostrocaudal polarity formation of the tectum"Dev.Growth Differ.. 46・3(in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakamura, H., Watanabe, Y.: "Isthmus organizer and regionalization of the mesencephalon and metencephalon"Int.J.Dev.Biol.. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakamura, H., Katahira, T., Watanabe, Y., Funahashi, J.: "Gain and loss of function in chick embroys by electroporation"Mech.Dev.. (in press).

    • Related Report
      2003 Annual Research Report
  • [Publications] 仲村春和(伊藤正男監修, 金澤一郎, 篠田義一, 広川信隆, 御子柴克彦, 宮下保司編): "脳神経科学"三輪書店. 841 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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