Cancer fluid biopsy for childhood malignancies using one cell analysis

• Project/Area Number: 15H02567
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Hiroshima University
• Principal Investigator: Hiyama Eiso 広島大学, 自然科学研究支援開発センター, 教授 (00218744)
• Co-Investigator(Kenkyū-buntansha): 金輪 真佐美 (福永真佐美) 広島大学, 自然科学研究支援開発センター, 助教 (00284208) ; 栗原 將 広島大学, 医歯薬保健学研究科(医), 助教 (40724894) ; 上田 祐華 広島大学, 病院(医), 病院助教 (70624641) ; 外丸 祐介 広島大学, 自然科学研究支援開発センター, 教授 (90309352)
• Co-Investigator(Renkei-kenkyūsha): MASUJIMA TSUTOMU 理化学研究所, 生命システム研究センター, チームリーダー (10136054)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000); Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2016: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2015: ¥27,170,000 (Direct Cost: ¥20,900,000、Indirect Cost: ¥6,270,000)
• Keywords: 癌 / 細胞 / 組織 / 核酸 / バイオテクノロジー / 臨床 / 細胞、組織

Outline of Final Research Achievements

Cancer liquid biopsy was performed on primary cultured cells, frozen specimens and plasma of pediatric tumors preserved in Hiroshima University Hospital and circulating tumor cells collected with magnetic beads cell concentrators. In single cell mass spectrometry (cellomics), anaerobic glycolysis was enhanced in cancer stem cells, and in single cell genomic mutations were detectable. Prognosis-related microRNAs were isolated from exosomes as a biomarker search in culture supernatant and plasma. Furthermore, it was also possible to detect gene mutations such as MYC gene amplification and β catenin mutation by the next generation sequencing and digital PCR using cell free DNA. It was considered to be a useful tool for minimally invasive diagnosis, drug susceptibility and monitoring of recurrence.

Research Products

• [Journal Article] Differentiated embryo chondrocyte plays a crucial role in DNA damage response via transcriptional regulation under hypoxic conditions (2018)
  • Author(s): Nakamura Hideaki、Bono Hidemasa、Hiyama Keiko、Kawamoto Takeshi、Kato Yukio、Nakanishi Takeshi、Nishiyama Masahiko、Hiyama Eiso、Hirohashi Nobuyuki、Sueoka Eisaburo、Poellinger Lorenz、Tanimoto Keiji
  • Journal Title: PLOS ONE Volume: 13 Issue: 2 Pages: e0192136-e0192136
  • DOI: 10.1371/journal.pone.0192136
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sodium Thiosulfate Protection of Cisplatin-Induced Ototoxicity (2018)
  • Author(s): Brock PR, Maibach R, Childs M, Rajput K, Roebuck D, Sullivan MJ, Laithier V, Ronghe M, Dall'Igna P, Hiyama E, Brichard B, Skeen J, Mateos ME, Capra M, Rangaswami AA, Ansari M, Rechnitzer C, Veal GJ, Covezzoli A, Brugieres L, Perilongo G, Czauderna P, Morland B, Neuwelt EA
  • Journal Title: New Eng. J. Med Volume: in press
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass. (2018)
  • Author(s): Yang B, Kumoto T, Arima T, Nakamura M, Sanada Y, Kumrungsee T, Sotomaru Y, Shimada M, Yanaka N.
  • Journal Title: Genes Cells Volume: 23 Issue: 3 Pages: 136-145
  • DOI: 10.1111/gtc.12558
  • Peer Reviewed / Open Access
• [Journal Article] Inflammatory myofibroblastic tumors of the lung carrying a chimeric A2M-ALK gene: report of 2 infantile cases and review of the differential diagnosis of infantile pulmonary lesions (2017)
  • Author(s): Tanaka Mio、Kohashi Kenichi、Kushitani Kei、Yoshida Misa、Kurihara Sho、Kawashima Masumi、Ueda Yuka、Souzaki Ryota、Kinoshita Yoshiaki、Oda Yoshinao、Takeshima Yukio、Hiyama Eiso、Taguchi Tomoaki、Tanaka Yukichi
  • Journal Title: Human Pathology Volume: 66 Pages: 177-182
  • DOI: 10.1016/j.humpath.2017.06.013
  • Peer Reviewed / Open Access
• [Journal Article] Risk-stratified staging in paediatric hepatoblastoma: a unified analysis from the Children's Hepatic tumors International Collaboration. (2017)
  • Author(s): Meyers RL, Maibach R, Hiyama E, Harberle B, Krailo M, Rangaswami A, Aronson DC, Malogolowkin MH, Perilongo G, von Schweinitz D, Ansari M, Lopez-Terrada D, Tanaka Y, Alaggio R, Leuschner I, Hishiki T, Schmid I, Watanabe K, Yoshimura K, Feng Y, Rinaldi E, Saraceno D, Derosa M, Czauderna P.
  • Journal Title: Lancet Oncol. Volume: 18 Issue: 1 Pages: 122-131
  • DOI: 10.1016/s1470-2045(16)30598-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The role of pulmonary metastasectomy for hepatoblastoma in children with metastasis at diagnosis: Results from the JPLT-2 study (2017)
  • Author(s): Hishiki T, Watanabe K, Ida K, Hoshino K, Iehara T, Aoki Y, Kazama T, Kihira K, Takama Y, Taguchi T, Fujimura J, Honda S, Matsumoto K, Mori M, Yano M, Yokoi A, Tanaka Y, Fuji H, Miyazaki O, Yoshimura K, Takimoto T, Hiyama E
  • Journal Title: Journal of Pediatric Surgery Volume: 52 Issue: 12 Pages: 2051-2055
  • DOI: 10.1016/j.jpedsurg.2017.08.031
  • Peer Reviewed
• [Journal Article] Overexpression of microRNAs from the Gtl2-Rian locus contributes to postnatal death in mice. (2017)
  • Author(s): Kumamoto S, Takahashi N, Nomura K, Fujiwara M, Kijioka M, Uno Y, Matsuda Y, Sotomaru Y, Kono T.
  • Journal Title: Hum Mol Genet Volume: 26 Issue: 19 Pages: 3653-3662
  • DOI: 10.1093/hmg/ddx223
  • Peer Reviewed / Open Access
• [Journal Article] 小児肝腫瘍の最近の話題：グローバル試験へ向けて (2016)
  • Author(s): 檜山 英三, 上田 祐華, 栗原 将, 河島 茉澄
  • Journal Title: 小児外科 Volume: 48 Pages: 1192-1195
  • Peer Reviewed
• [Journal Article] Clinical prognostic value of DNA methylation in hepatoblastoma: Four novel tumor suppressor candidates (2016)
  • Author(s): Honda S, Minato M, Suzuki H, Fujiyoshi M, Miyagi H, Haruta M, Kaneko Y, Hatanaka KC, Hiyama E, Kamijo T, Okada T, Taketomi A
  • Journal Title: Cancer Science Volume: in press Issue: 6 Pages: 812-819
  • DOI: 10.1111/cas.12928
  • NAID: 120005828475
  • Peer Reviewed / Open Access
• [Journal Article] Prognostic value of circulating tumour DNA in patients undergoing curative resection for pancreatic cancer. (2016)
  • Author(s): Hadano N, Murakami Y, Uemura K, Hashimoto Y, Kondo N, Nakagawa N, Sueda T, Hiyama E.
  • Journal Title: Br J Cancer Volume: 175 Issue: 1 Pages: 69-75
  • DOI: 10.1038/bjc.2016.175
  • Peer Reviewed
• [Journal Article] Serum-Based Quantification of MYCN Gene Amplification in Young Patients with Neuroblastoma: Potential Utility as a Surrogate Biomarker for Neuroblastoma. (2016)
  • Author(s): 4.Yagyu S, Iehara T, et al.
  • Journal Title: PLoS One. Volume: 11(8) Issue: 8 Pages: e0161039-e0161039
  • DOI: 10.1371/journal.pone.0161039
  • NAID: 120005997753
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Resectability and tumor response after preoperative chemotherapy in hepatoblastoma treated by the Japanese Study Group for Pediatric Liver Tumor (JPLT)-2 protocol. (2016)
  • Author(s): Hiyama E, Hishiki T, Watanabe K, Ida K, Yano M, Oue T, Iehara T, Hoshino K, Koh K, Tanaka Y, Kurihara S, Ueda Y, Onitake Y.
  • Journal Title: J Pediatr Surg. Volume: 51 Issue: 12 Pages: 2053-2057
  • DOI: 10.1016/j.jpedsurg.2016.09.038
  • Peer Reviewed
• [Journal Article] Telomere biology including TERT rearrangements in neuroblastoma: a useful indicator for surgical treatments (2016)
  • Author(s): Kawashima Masumi、Kojima Masato、Ueda Yuka、Kurihara Sho、Hiyama Eiso
  • Journal Title: Journal of Pediatric Surgery Volume: 51 Issue: 12 Pages: 2080-2085
  • DOI: 10.1016/j.jpedsurg.2016.09.042
  • Peer Reviewed
• [Journal Article] Transcriptional regulator Bhlhe40 works as a cofactor of T-bet in the regulation of IFN-γ production in iNKT cell (2016)
  • Author(s): Kanda M, Yamanaka H, Kojo S, Usui Y, Honda H, Sotomaru Y, Harada M, Taniguchi M, Suzuki N, Atsumi T, Wada H, Bagdadi M, Seino, K
  • Journal Title: Proceedings of the National Academy of Sciences of the USA Volume: 14: 113(24) Issue: 24
  • DOI: 10.1073/pnas.1604178113
  • Peer Reviewed / Open Access
• [Journal Article] KRAS mutation leads to decreased expression of regulator of calcineurin 2, resulting in tumor proliferation in colorectal cancer. (2016)
  • Author(s): Niitsu H, Hinoi T, Kawaguchi Y, Sentani K, Yuge R, Kitadai Y, Sotomaru Y, Adachi T, Saito Y, Miguchi M, Kochi M, Sada H, Shimomura M, Oue N, Yasui W, Ohdan H.
  • Journal Title: Oncogenesis. Volume: 5 Issue: 8 Pages: 8-8
  • DOI: 10.1038/oncsis.2016.47
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gasdermin C Is Upregulated by Inactivation of Transforming Growth Factor β Receptor Type II in the Presence of Mutated Apc, Promoting Colorectal Cancer Proliferation (2016)
  • Author(s): Miguchi M, Hinoi T, Shimomura M et al
  • Journal Title: PloS One Volume: 11 Issue: 11 Pages: 0166422-0166422
  • DOI: 10.1371/journal.pone.0166422
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DEC2 is a negative regulator for the proliferation and differentiation of chondrocyte lineage-committed mesenchymal stem cells (2016)
  • Author(s): Sasamoto, T., Fujimoto, K., Kanawa, M., Kimura, J., Takeuchi, J., Harada, N., Goto, N., Kawamoto, T., Noshiro, M., Suardita, K., Tanne, K., Kato, Y.
  • Journal Title: Int. J. Mol. Med Volume: 印刷中 Issue: 3 Pages: 876-884
  • DOI: 10.3892/ijmm.2016.2660
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High glucose promotes TGF-β1 production by inducing FOS expression in human peritoneal mesothelial cells (2016)
  • Author(s): Kokoroishi K, Nakashima A, Doi S, Ueno T, Doi T, Yokoyama Y, Honda K, Kanawa M, Kato Y, Kohno N, Masaki T.
  • Journal Title: Clin Exp Nephrol. Volume: 20 Issue: 1 Pages: 30-38
  • DOI: 10.1007/s10157-015-1128-9
  • Peer Reviewed
• [Journal Article] Chlorinated Water Modulates the Development of Colorectal Tumors with Chromosomal Instability and Gut Microbiota in Apc-Deficient Mice. (2015)
  • Author(s): Sasada T, Hinoi T, Saito Y, Adachi T, Takakura Y, Kawaguchi Y, Sotomaru Y, Sentani K, Oue N, Yasui W, Ohdan H.
  • Journal Title: PLoS One. Volume: 10 Issue: 7 Pages: e0132435-e0132435
  • DOI: 10.1371/journal.pone.0132435
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells (2015)
  • Author(s): Fujii S, Fujimoto K, Goto N, Kanawa M, Kawamoto T, Pan H, Srivatanakul P, Rakdang W, Pornprasitwech J, Saskianti T, Suardita K, Nishimura F, Kato Y.
  • Journal Title: Free PMC Article Volume: 3 Pages: 566-572
  • Peer Reviewed
• [Journal Article] Circulating free DNA as non-invasive diagnostic biomarker for childhood solid tumors (2015)
  • Author(s): Kurihara S, Ueda Y, Onitake Y, Sueda T, Ohta E, Morihara N, Hirano S, Irisuna F, Hiyama E
  • Journal Title: Journal of Pediatric Surgery Volume: 50 Issue: 12 Pages: 2094-2097
  • DOI: 10.1016/j.jpedsurg.2015.08.033
  • Peer Reviewed / Open Access
• [Journal Article] Mortality and morbidity in primarily resected hepatoblastomas in Japan: Experience of the JPLT (Japanese Study Group for Pediatric Liver Tumor) trials (2015)
  • Author(s): Hiyama E, Hishiki T, Watanabe K, Ida K, Yano M, Oue T, Iehara T, Hoshino K, Koh K, Tanaka Y, Kurihara S, Ueda Y, Onitake Y
  • Journal Title: Journal of Pediatric Surgery Volume: 50 Issue: 12 Pages: 2098-2101
  • DOI: 10.1016/j.jpedsurg.2015.08.035
  • Peer Reviewed / Open Access
• [Journal Article] Direct Lipido-Metabolomics of Single Floating Cells for Analysis of Circulating Tumor Cells by Live Single-cell Mass Spectrometry (2015)
  • Author(s): Hiyama E, Ali A, Amer S, Harada T, Shimamoto K, Furushima R, Abouleila Y, Emara S, Masujima T
  • Journal Title: Analytical Sciences Volume: 31 Issue: 12 Pages: 1215-1217
  • DOI: 10.2116/analsci.31.1215
  • NAID: 130005129718
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Children's Hepatic tumors International Collaboration (CHIC): Novel global rare tumor database yields new prognostic factors in hepatoblastoma and becomes a research model (2015)
  • Author(s): Czauderna P, Haeberle B, Hiyama E, Rangaswami A, Krailo M, Maibach R, Rinaldi E, Feng Y, Aronson D, Malogolowkin M, Yoshimura K, Leuschner I, Lopez-Terrada D, Hishiki T, Perilongo G, von Schweinitz D, Schmid I, Watanabe K, Derosa M, Meyers R
  • Journal Title: European Journal of Cancer Volume: 1 Pages: 92-101
  • DOI: 10.1016/j.ejca.2015.09.023
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nerve Growth Factor Expression Is Not Associated with Perineural Invasion in Extrahepatic Cholangiocarcinoma (2015)
  • Author(s): Urabe K, Murakami Y, Kondo N, Uemura K, Hashimoto Y, Nakagawa N, Sasaki H, Hiyama E, Takahashi S, Sueda T.
  • Journal Title: Digestive Diseases and Sciences Volume: 61 Issue: 3 Pages: 774-784
  • DOI: 10.1007/s10620-015-3953-9
  • Peer Reviewed / Int'l Joint Research
• [Presentation] PULMONARY METASTASECTOMY IN HEPATOBLASTOMA-JPLT-2 EXPEROENCE (2017)
  • Author(s): Hishiki T, Watanabe K, Ida K, Hoshuno K, Iehara T, Aoki Y, Kazama T, Kihira K, Takama Y, Taguchi T, Fujimura J, Honda S, Matsumoto K, Mori M, Yano M, Yokoi A, Tanaka Y, Fuji H, Miyazaki O, Yoshimura K, Takimoto T, Hiyama E
  • Organizer: SIOPEL SPRING MEETING 2017
  • Int'l Joint Research
• [Presentation] 肝芽腫の包括的メチル化解析で明らかとなった予後不良に関連するメチル化サブタイプと癌特異的エンハンサー (2017)
  • Author(s): 永江 玄太, 山本 尚吾, 辰野 健二, Claire Renard-Guillet, 梅田 高呂, 堤 修一, 藤田 征志, 中川 英刀, 檜山 英三, 油谷 浩幸
  • Organizer: 第76回日本癌学会学術集会
• [Presentation] 肝芽腫におけるシスプラチン耐性関連遺伝子の異常高メチル化の同定 (2017)
  • Author(s): 藤好, 直, 本多 昌平, 湊 雅嗣, 鈴木 拓, 檜山 英三, 武冨 紹信
  • Organizer: 第76回日本癌学会学術集会
• [Presentation] DHRS3 とNR0B1 は神経芽腫細胞株の分化と増殖抑制を誘導する (2017)
  • Author(s): 山岡 絵美, 久保 陽子, 檜山 英三
  • Organizer: 第76回日本癌学会学術集会
• [Presentation] Integrated Genetic and Epigenetic Analysis novel Molecular Subgroups in Hepatoblastoma (2017)
  • Author(s): Hiyama E, Kurihara S, Hirano S, Irisuna F, Ueda Y, Kawashima M
  • Organizer: The 49th Congress of the International Society of Paediatric Oncology (SIOP2017)
  • Int'l Joint Research
• [Presentation] The probability of liquid biopsy as non-invasive diagnostic tool for childhood solid tumors (2017)
  • Author(s): Kurihara S, Hiyama E, Ueda Y
  • Organizer: 第59回日本小児血液・がん学会学術集会
• [Presentation] 両側多発性腎腫瘍の一例 (2017)
  • Author(s): 野間 康輔, 望月 慎史, 江口 勇太, 小野 大地, 松村 梨沙, 土居 岳彦, 川口 浩史, 栗原 将, 檜山 英三, 小林 正夫
  • Organizer: 第59回日本小児血液・がん学会学術集会
• [Presentation] 小児肝がん細胞において高発現する膜蛋白ADAM32の意義 (2017)
  • Author(s): 深澤 賢宏, 谷本 圭司, 山岡 絵美, 金輪 真佐美, 廣橋 伸之, 檜山 英三
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] Exome sequencing of hepatoblastoma identifies biological signatures and protential therapeutic targets (2016)
  • Author(s): Hiyama E
  • Organizer: International Pediatric Liver Tumor Symposium
  • Place of Presentation: Texas, USA.
  • Year and Date: 2016-02-25
  • Int'l Joint Research / Invited
• [Presentation] 肝芽腫において異常メチル化で発現抑制されるPARP6の機能解析 (2016)
  • Author(s): 本多 昌平, 宮城 久之, 檜山 英三, 武冨 紹信
  • Organizer: 第116回日本外科学会定期学術集会
• [Presentation] 膵癌患者におけるcirculating tumor DNAの検出と予後 (2016)
  • Author(s): 村上 義昭, 上村 健一郎, 橋本 泰司, 近藤 成, 中川 直哉, 末田 泰二郎, 檜山 英三
  • Organizer: 第116回日本外科学会定期学術集会
• [Presentation] Telomere biology in neuroblastoma: focusing on alteration of TERT promoter lesion (2016)
  • Author(s): Hiyama E, Kawashima M, Kurihara S, Ueda Y, Onitake Y, Hiyama K
  • Organizer: Advances in Neuroblastoma Research Congress (ANR) 2016
  • Int'l Joint Research
• [Presentation] Incidence of stage IV neuroblastoma patients 2-5 years of age was increased after the cessation of mass screening in Japan (2016)
  • Author(s): Yoneda A, Tajiri T, Hiyama E, Iehara T, Hishiki T, Kinoshita Y, Yonekura T, Yokota I, Teramukai S
  • Organizer: Advances in Neuroblastoma Research Congress (ANR) 2016
  • Int'l Joint Research
• [Presentation] 予後良好な神経芽細胞腫で発現していた3 遺伝子の機能解 (2016)
  • Author(s): 山岡 絵美, 林 陽子, 檜山 英三
  • Organizer: 第75回日本癌学会学術総会
• [Presentation] 神経芽腫細胞株における分化誘導候補因子の解析 (2016)
  • Author(s): 山岡 絵美, 金輪 真佐美, 田川 浩美, 福場 郁子, 久保 陽子, 古屋敷 なぎさ, 平野 尚子, 深澤 賢宏, 檜山 英三
  • Organizer: 第39回日本分子生物学会年会
• [Presentation] Cancer Fluid Biopsy Using Circulating Tumour Cells and Circulating Free DNA in Childhood Solid Tumors (2016)
  • Author(s): Hiyama E, Kurihara S, Ueda Y, Kawashima M, Hiyama K
  • Organizer: 48th Congress of the International Society of Paediatric Oncology (SIOP)
  • Int'l Joint Research
• [Presentation] 当院における小児腎腫瘍18例の検討 (2016)
  • Author(s): 兒島 正人, 河島 茉澄, 栗原 將, 上田 祐華, 檜山 英三
  • Organizer: 第58回日本小児血液・がん学会学術集会
• [Presentation] 後腹膜原発peripheral primitive neuroectodermal tumor(pPNET)の1例 (2016)
  • Author(s): 栗原 將, 上田 祐華, 河島 茉澄, 溝口 洋子, 今中 雄介, 下村 麻衣子, 川口 浩史, 小林 正夫, 檜山 英三
  • Organizer: 第58回日本小児血液・がん学会学術集会
• [Presentation] 様々な核の組合せにより作製したマーモセット４倍体胚の発生能について (2016)
  • Author(s): 外丸祐介、信清麻子、吉岡みゆき、畠山照彦、黒滝陽子、平川玲子、佐々木えりか
  • Organizer: 日本マーモセット研究会
• [Presentation] Effect of Mesenchymal Stem Cells on Mixed Self Assembled Monolayer (2016)
  • Author(s): Hirata I, Veronica SR, Tania S, Yamauchi Y, Kanawa M, Kato Y, Kato K
  • Organizer: The International Dental Materials Congress 2016 (Bali, Indonesia)
  • Int'l Joint Research
• [Presentation] 予後良好な神経芽細胞腫で高発現していたDHRS3, NR0B1, CYP26A1の機能解析 (2015)
  • Author(s): 山岡 絵美, 金輪 真佐美, 田川 浩美, 福場 郁子, 久保-林 陽子, 古屋敷 なぎさ, 阿部 裕子, 平野 尚子, 檜山 英三
  • Organizer: BMB2015（第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会）
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2015-12-01
• [Presentation] 小児肝がんに対するゲノム解析と国際共同臨床試験へのアプローチ (2015)
  • Author(s): 檜山 英三
  • Organizer: 第53回日本癌治療学会学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2015-10-29
  • Int'l Joint Research
• [Presentation] 霊長類受精卵のガラス化保存 (2015)
  • Author(s): 外丸祐介, 信清麻子, 吉岡みゆき，畠山照彦，印藤頼子，兼子明久，岡本宗裕，岡原純子，佐々木えりか
  • Organizer: Cryopreservation Conference 2015
  • Place of Presentation: 愛知県岡崎市
  • Year and Date: 2015-10-28
• [Presentation] シングルセル解析を目指した血中循環腫瘍細胞（CTC ： Circullating Tumor Cells）の基礎的研究について (2015)
  • Author(s): 島本 和美, 檜山 英三
  • Organizer: 第74回日本癌学会学術集会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2015-10-08
• [Presentation] Integrated exome analysis in childhood hepatoblastoma: Biological approach for molecular targeting (2015)
  • Author(s): Hiyama E, Kurihara S, Onitake Y, Ueda Y, Morihara N, Fukuba I, Komatsu R.
  • Organizer: SIOP 2015
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2015-10-08
  • Int'l Joint Research
• [Presentation] フローサイトメーターによる血中循環腫瘍細胞(CTC)の測定について（神経芽腫） (2015)
  • Author(s): 島本 和美, 檜山 英三
  • Organizer: 第25回日本サイトメトリー学会学術集会
  • Place of Presentation: 東京都
  • Year and Date: 2015-07-11
• [Presentation] Survival and late effects in the risk-stratified hepatoblastoma patients treated by JPLT-2 protocol (2015)
  • Author(s): Hiyama E, Kurihara S, Ueda Y, Hishiki T, Ida K, Iehara T, Oue T, Yano M, Watanabe K, Hoshino K, Ko K, Tanaka Y.
  • Organizer: 2015 ASCO Annual Meeting
  • Place of Presentation: Chicago, U.S.A
  • Year and Date: 2015-05-29
  • Int'l Joint Research
• [Presentation] コモンマーモセット凍結保存精子の運動性と体外受精能 (2015)
  • Author(s): 外丸祐介、畠山照彦、吉岡みゆき、信清麻子
  • Organizer: 第62回日本実験動物学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2015-05-28
• [Presentation] OCIAD2はDNAメチル化により制御される肝芽腫の新規癌抑制遺伝子である (2015)
  • Author(s): 湊 雅嗣, 本多 昌平, 小林 希, 三次 早香, 鈴木 拓, 岡田 忠雄, 宮城 久之, 檜山 英三, 武冨 紹信
  • Organizer: 第52回日本小児外科学会学術集会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2015-05-28
• [Presentation] Circulating free DNA as non-invasive diagnostic biomarker for childhood solid tumors (2015)
  • Author(s): Kurihara S, Ueda Y, Onitake Y, Sueda T, Ohta E, Morihara N, Hirano S, Irisuna F, Hiyama E.
  • Organizer: 48th Annual Meeting of the Pacific Association of Pediatric Surgeons
  • Place of Presentation: Jeju, South Kore
  • Year and Date: 2015-05-17
  • Int'l Joint Research
• [Presentation] Mortality and morbidity in primarily resected hepatoblastomas in Japan: experience of JPLT (Japanese study group for pediatric liver tumor) trials (2015)
  • Author(s): Hiyama E, Hishiki T, Watanabe K, Ida K, Yano M, Oue T, Iehara T, Hoshino K, Koh K, Tanaka Y, Kurihara S, Ueda Y, Onitake Y.
  • Organizer: 48th Annual Meeting of the Pacific Association of Pediatric Surgeons
  • Place of Presentation: Jeju, South Kore
  • Year and Date: 2015-05-17
  • Int'l Joint Research
• [Presentation] Droplet Digital PCRを用いた膵癌KRAS遺伝子変異の研究 (2015)
  • Author(s): 羽田野 直人, 村上 義昭, 上村 健一郎, 橋本 泰司, 近藤 成, 中川 直哉, 佐々木 勇人, 檜山 英三, 末田 泰二郎
  • Organizer: 第115回日本外科学会定期学術集会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2015-04-16
• [Book] 標準小児外科学 第7版 (2017)
  • Author(s): 檜山 英三
  • Total Pages: 7
  • Publisher: 医学書院
  • ISBN: 4260027808
• [Book] よくわかる臨床研究～小児がん～ 小児がん臨床研究プロトコルの実際「肝腫瘍」 (2016)
  • Author(s): 檜山 英三
  • Total Pages: 6
  • Publisher: 医薬ジャーナル社
• [Book] 小児血液・腫瘍学 (2015)
  • Author(s): 檜山 英三
  • Total Pages: 585
  • Publisher: 診断と治療社