Microbial arsenic transformation pathways associated with polluted environments

• Project/Area Number: 15H02842
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Modeling and technologies for environmental conservation and remediation
• Research Institution: Kyushu University
• Principal Investigator: Hamamura Natsuko 九州大学, 理学研究院, 准教授 (50554466)
• Co-Investigator(Kenkyū-buntansha): 光延 聖 愛媛大学, 農学研究科, 准教授 (70537951)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: ヒ素 / 微生物ヒ素酸化 / 土壌微生物 / ヒ素酸化酵素 / 微生物ヒ素代謝 / アンチモン / 汚染土壌 / 嫌気的ヒ素酸化 / 土壌ヒ素汚染 / 環境技術 / 生態系修復 / ヒ素汚染 / 土壌汚染防止・浄化 / 汚染物質動態とモデリング / 微生物酸化還元反応 / 微生物生態学 / 地球微生物学 / 汚染質動態とモデリング

Outline of Final Research Achievements

In this study, the influence of environmental factors on biotransformation of arsenic by soil microbial community was investigated. Microbial arsenite oxidation was detected in the soil columns under oxic and anoxic conditions concomitant with the emergence of arsenite-oxidizing bacterial population and increase in arsenite oxidase gene diversity. Our results also indicated that the co-contamination of toxic metals along with arsenic imposed a selective pressure on the soil bacterial community and thus affecting the redox transformation of arsenic in natural environments.

Academic Significance and Societal Importance of the Research Achievements

ヒ素汚染の原因究明は世界的にも注視されているが、ヒ素と微生物の相互作用は未解明な部分が多いため、実際の汚染土壌に有効な管理・浄化技術が確立されておらず、安全性を評価し二次汚染被害拡大を阻止する対策が求められている。本研究の成果は、環境中ヒ素挙動における生物学的な関与を明らかにするとともに、汚染土壌中でヒ素形態変化に関与する新規微生物群の取得により、ヒ素汚染リスク低減技術や評価技術への応用が期待できる。また、本研究で実施した環境中での微生物群の活性全体を定量的に検出・測定する手法はヒ素以外の化学物質代謝へ応用も期待されることから、学術的な意義も高い。

Research Products

• [Int'l Joint Research] Texas A&M University(U.S.A.)
• [Journal Article] Millimeter-scale topsoil layer blocks arsenic migration in flooded paddy soil (2020)
  • Author(s): Mitsunobu, S., Toda, M., Hamamura, N., Shiraishi, F., Tominaga, Y., Sakata, M.
  • Journal Title: Geochimica et Cosmochimica Acta Volume: 274 Pages: 211-227
  • DOI: 10.1016/j.gca.2020.01.038
  • Peer Reviewed
• [Journal Article] Expression of genes and proteins involved in arsenic respiration and resistance in dissimilatory arsenate-reducing Geobacter sp. strain OR-1. (2019)
  • Author(s): Tsuchiya, T., Ehara, A., Kasahara, Y., Hamamura, N., Amachi, S.
  • Journal Title: Applied and Environmnetal Microbiology Volume: 85 Issue: 14
  • DOI: 10.1128/aem.00763-19
  • Peer Reviewed
• [Journal Article] The influence of chemical form of antimony on soil microbial community structure and arsenite oxidation activity (2018)
  • Author(s): Kataoka, T., Mitsunobu, S., Hamamura, N.
  • Journal Title: Microbes and Environment Volume: 33 Pages: 214-221
  • NAID: 130007393735
  • Peer Reviewed / Open Access
• [Journal Article] Cellular response of Sinorhizobium sp. Strain A2 associated with arsenite oxidation. (2015)
  • Author(s): Fukushima, K., Huang, H., Hamamura, N.
  • Journal Title: Microbe and Environment Volume: 30 Issue: 4 Pages: 330-334
  • DOI: 10.1264/jsme2.me15096
  • NAID: 130005116035
  • Peer Reviewed / Open Access
• [Presentation] Functional analysis of microbial communities associated with arsenic transformation in contaminated soil. (2019)
  • Author(s): Hamamura, N., Kataoka, T.
  • Organizer: The 11th Asian Symposium on Microbial Ecology
  • Int'l Joint Research
• [Presentation] Diversity of microbial arsenic and antimony transformation pathways associated with antimony mine tailing (2019)
  • Author(s): Hamamura, N., Yamashita, Y., Kataoka, T., Mitsunobu, S.
  • Organizer: Goldschmidt2019
  • Int'l Joint Research
• [Presentation] ヒ素の環境挙動に影響を及ぼす微生物代謝機構の多様性 (2019)
  • Author(s): 濱村奈津子
  • Organizer: 資源・素材学会2019
  • Invited
• [Presentation] Microbial Biotransformation of Toxic Metalloids and Its Bioremediation Potentials (2019)
  • Author(s): Hamamura, N., Okubo, T.,Mitsunobu, S.
  • Organizer: International Conference on Materials and Systems for Sustainability 2019
  • Int'l Joint Research
• [Presentation] Microbial community function and response associated with metalloid redox transformations in the contaminated environment (2017)
  • Author(s): Hamamura, N., Kataoka, T.
  • Organizer: JpGU-AGU Joint Meeting 2017
  • Int'l Joint Research
• [Presentation] Microbial biotransformation of toxic metalloids and its bioremediation implications (2017)
  • Author(s): Hamamura, N., Mitsunobu, S.
  • Organizer: The 14th International Symposium on Persistent Toxic Substances
  • Int'l Joint Research
• [Presentation] 環境撹乱に対する微生物群集応答のダイナミクス：群集レベルの機能発現と遺伝子多様性をつなぐ観点からのアプローチ (2017)
  • Author(s): 濱村奈津子
  • Organizer: 進化群集生態学シンポジウム2017
  • Invited
• [Presentation] Diversity of microbial metalloid transformation pathways and its geochemical implications (2016)
  • Author(s): Hamaura, N., Mitsunobu, S.
  • Organizer: Japan Geoscience Union Meeting 2016
• [Presentation] Diversity of microbial arsenic transformation pathways and community response to arsenic in a saline lake habitat (2016)
  • Author(s): Hamamura, N., Huang, H., Damdinsuren, N.
  • Organizer: The 8th Asian Symposium on Microbial Ecology
  • Int'l Joint Research
• [Presentation] 土壌環境のヒ素挙動に関与する微生物群集機能の解明 (2016)
  • Author(s): 片岡 剛文、濱村奈津子
  • Organizer: 日本微生物生態学会第31会大会
• [Presentation] Microbial antimony transformation associated with antimony mine tailing (2016)
  • Author(s): Hamaura, N., Utsunomiya, S., Nakano, Y., Mitsunobu, S.
  • Organizer: Goldschmidt2016
  • Int'l Joint Research
• [Presentation] Diversity of microbial arsenic transformation pathways associated with arsenic impacted environments (2015)
  • Author(s): Hamamura, N.
  • Organizer: 4th Internationa Congress on Natural Science
  • Place of Presentation: Changhua, Taiwan
  • Year and Date: 2015-09-10
  • Int'l Joint Research
• [Presentation] Microbial transformation of antimony and its geochemical implications (2015)
  • Author(s): Hamamura, N., Mori, K., Mitsunobu, S.
  • Organizer: Japan Geoscience Union Meeting 2015
  • Place of Presentation: 幕張
  • Year and Date: 2015-05-25