| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
We examined possibilities that new methods could be introduced into Quaternary paleoenvironment reconstruction using DNA analysis of pollen fossils and oxygen isotope ratio of phytolith fossils. We examined PCR amplification conditions for chloroplast genome analysis using modern conifers as test samples. Although we could obtain primers suitable for analysis of phylogenetic comparison evolution above the genus level, we could not find the amplification condition suitable for species identification from total DNA samples. Further examination of amplification conditions is necessary for the DNA analysis of pollen fossils, which are anticipated to decrease and fragment the DNA content itself due to aging. Since differences in oxygen isotope ratio analysis results by different methods were recognized for phytoliths extracted from modern plants, it is necessary to establish a routine way to measure the oxygen isotope ratio of phytoliths with standard samples.
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