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Structural analysis of membrane protein based on multi-labeled sites

Research Project

Project/Area Number 15H03119
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemical biology
Research InstitutionUniversity of Toyama

Principal Investigator

TOMOHIRO Takenori  富山大学, 大学院医学薬学研究部(薬学), 教授 (70357581)

Co-Investigator(Kenkyū-buntansha) 千葉 順哉  富山大学, 大学院医学薬学研究部(薬学), 助教 (50436789)
Co-Investigator(Renkei-kenkyūsha) SHIMIZU Takahiro  富山大学, 大学院医学薬学研究部(薬学), 准教授 (40353437)
MATSUNAGA Shigeru  浜松ホトニクス株式会社, 中央研究所, 専任部員 (40391198)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords化学プローブ / 光アフィニティーラベル / 結合部位解析 / 分子認識 / 標的同定 / バイオテクノロジー / ケミカルバイオロジー / プロテオミクス
Outline of Final Research Achievements

Multifunctionalization of photocross-linker including fluorogenicity and cleavability has improved efficiency of labeled site analysis and enabled simultaneous analysis of multiple trace amount labels. We have developed a new methodology to monitor the allosteric structural change of protein due to binding of substrates based on label amount change of each labeled amino acid within the ligand-binding domain.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (23 results)

All 2018 2017 2016 2015 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] 薬の標的を同定し結合部位を解析する光技術2018

    • Author(s)
      友廣岳則
    • Journal Title

      光アライアンス

      Volume: 28

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Simple Synthesis of Sulfonyl Amidine-Containing Glucosidase Inhibitors by a Chemoselective Coupling Reaction Between D-Gluconothiolactam and Sulfonyl Azides2017

    • Author(s)
      Aswad Muhammad、Chiba Junya、Tomohiro Takenori、Hatanaka Yasumaru
    • Journal Title

      International Research Journal of Pure and Applied Chemistry

      Volume: 14 Issue: 2 Pages: 1-8

    • DOI

      10.9734/irjpac/2017/34259

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Kinetic controlled affinity labeling of target enzyme with thioester chemistry2016

    • Author(s)
      Tomohiro, T.; Nakabayashi, M.; Sugita, Y.; Morimoto, S.
    • Journal Title

      Bioorganic Medicinal Chemistry

      Volume: 24 Issue: 15 Pages: 3336-3341

    • DOI

      10.1016/j.bmc.2016.05.059

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Design and preparation of a compact multifunctional diazirine-based photocross-linker2016

    • Author(s)
      Yasui, S.; Morimoto, S.; Chiba, J.; Hatanaka, Y.; Tomohiro, T.
    • Journal Title

      Photomedicine and Photobiology

      Volume: 38 Pages: 7-8

    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Structure-assisted ligand-binding analysis using fluorogenic photoaffinity labeling2015

    • Author(s)
      Masuda, S., Tomohiro, T., Yamaguchi, S., Morimoto, S., Hatanaka, Y.
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 25 Issue: 8 Pages: 1675-1678

    • DOI

      10.1016/j.bmcl.2015.03.008

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] アシルスルホンアミド型光反応基によるラベルタンパク質解析の効率化2018

    • Author(s)
      林龍二,森本正大,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 光切断性クマリン型クロスリンカーを用いた標的タンパク質同定戦略2017

    • Author(s)
      金子司,堀田侑佑,森本正大,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 発蛍光性光アフィニティーラベル法を用いたATP結合構造解析2017

    • Author(s)
      山口麗央奈,山口昇太,増田宗太,森本正大,千葉順哉,畑中保丸,友廣岳則.
    • Organizer
      日本ケミカルバイオロジー学会第12回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 標的タンパク質同定を目的とする切断性蛍光ラベル法の開発2017

    • Author(s)
      金子司,堀田侑佑,森本正大,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      第39回日本光医学・光生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] スルホクリック反応を利用した光反応性プローブの簡便合成と標的タンパク質同定の効率化2017

    • Author(s)
      林龍二,森本正大,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      日本プロテオーム学会2017年大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] PAL-based fluorogenic tagging for structural analysis of ligand binding state within target protein2017

    • Author(s)
      Tomohiro T, Yamaguchi, R, Morimoto S, Chiba J, Hatanaka Y.
    • Organizer
      26th French-Japanese Symposium on Medicinal&Fine Chemistry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] PeT-based photochemical fluorophore labelling for target identification2016

    • Author(s)
      Kaneko, T.; Hotta, Y.; Morimoto. S.; Chiba, J.; Hatanaka, Y.; Tomohiro, T.
    • Organizer
      The First International Symposium on Toyama-Asia-Africa Pharmaceutical Network
    • Place of Presentation
      Toyama
    • Year and Date
      2016-09-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 発蛍光性光ラベルのための小分子ユニットの開発2016

    • Author(s)
      安井翔,森本正大,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      第38回日本光医学・光生物学会
    • Place of Presentation
      京都
    • Year and Date
      2016-07-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] PeTを利用した光アフィニティー蛍光ラベル法の開発2016

    • Author(s)
      金子司,堀田侑佑,森本正大,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      日本ケミカルバイオロジー学会第11回年会;2016 Jun 15-17;
    • Place of Presentation
      京都
    • Year and Date
      2016-06-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 光切断特性を有する光クロスリンカーの開発と標的タンパク質蛍光ラベル化への応用2015

    • Author(s)
      堀田侑佑,山本章人,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      日本薬学会北陸支部第127回例会
    • Place of Presentation
      富山
    • Year and Date
      2015-11-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Photoaffinity labeling-based target identification of bioactive molecules2015

    • Author(s)
      Tomohiro, T.
    • Organizer
      The 2nd International Current Breakthrough (ICB)-Pharma Symposium
    • Place of Presentation
      Solo, Indonesia
    • Year and Date
      2015-10-31
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光切断特性を有する発蛍光性光クロスリンカーの開発2015

    • Author(s)
      堀田侑佑,山本章人,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      第9回バイオ関連化学シンポジウム
    • Place of Presentation
      熊本
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 多機能光アフィニティーラベル法による高速標的同定2015

    • Author(s)
      友廣岳則,森本正大,千葉順哉,畑中保丸
    • Organizer
      日本プロテオーム学会2015年会
    • Place of Presentation
      熊本
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光化学的蛍光性質量差タグ導入による高速標的同定2015

    • Author(s)
      森本正大,嶋 俊弥,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      第81回北陸質量分析談話会
    • Place of Presentation
      福井
    • Year and Date
      2015-06-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] 光化学的蛍光ラベル法によるATP結合構造の解析2015

    • Author(s)
      山口昇太,増田宗太,森本正大,千葉順哉,畑中保丸,友廣岳則
    • Organizer
      第81回北陸質量分析談話会
    • Place of Presentation
      福井
    • Year and Date
      2015-06-06
    • Related Report
      2015 Annual Research Report
  • [Book] Tag-Creation Approaches for Highly Efficient Profiling of Interacting Proteins and Domains. "Photoaffinity Labeling for Structural Probing within Protein" eds Y. Hatanaka, M. Hashimoto, Chapter 42017

    • Author(s)
      Takenori Tomohiro
    • Total Pages
      31
    • Publisher
      Springer International Publishing
    • ISBN
      9784431565697
    • Related Report
      2017 Annual Research Report
  • [Remarks] 生体認識化学研究室ホームページ

    • URL

      http://www.pha.u-toyama.ac.jp/anachem/anachem/homu.html

    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] 光切断性蛍光標識プローブ2015

    • Inventor(s)
      友廣岳則,堀田侑佑
    • Industrial Property Rights Holder
      富山大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-167367
    • Filing Date
      2015-08-27
    • Related Report
      2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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