Development of next-generation wireless-electroless oscillator biosensors for diagnosis and drug development

• Project/Area Number: 15H03587
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General applied physics
• Research Institution: Osaka University
• Principal Investigator: Ogi Hirotsugu 大阪大学, 工学研究科, 教授 (90252626)
• Co-Investigator(Kenkyū-buntansha): 南野 哲男 香川大学, 医学部, 教授 (30379234) ; 後藤 祐児 大阪大学, たんぱく質研究所, 教授 (40153770) ; 中村 暢伴 大阪大学, 基礎工学研究科, 助教 (50452404)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000); Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: QCM / バイオセンサー / 蛋白質相互作用 / 無線 / QCMバイオセンサー / MEMSプロセス / アミロイド線維 / 計測工学 / マイクロ・ナノデバイス / 核酸 / 脳神経疾患 / アミロイドβ / 超音波

Outline of Final Research Achievements

We have developed MEMS quartz-crystal-microbalance biosensors and succeeded in improving the stability and sensitivity. The high-frequency biosensors allowed us to detect target proteins even in a solution with impurity proteins whose concentrations were much higher than that of target protein. The viscoelasticity was successfully monitored during the structural transduction of Amyloid beta peptide using higher vibrational modes. Furthermore, combination of the wireless-electrodeless QCM in the total internal reflection fluorescence microscopy revealed novel properties of fibrillation of the papeitdes.

Research Products

• [Journal Article] Principle and Applications of Wireless Quartz-crystal-microbalance Biosensors (2018)
  • Author(s): 荻 博次
  • Journal Title: IEICE ESS Fundamentals Review Volume: 11 Issue: 3 Pages: 180-185
  • DOI: 10.1587/essfr.11.3_180
  • NAID: 130006301312
  • ISSN: 1882-0875
  • Peer Reviewed
• [Journal Article] RNA Aptamer Binds Heparin-Binding Epidermal Growth Factor-Like Growth Factor with High Affinity and Specificity and Neutralizes Its Activity. (2017)
  • Author(s): Masaki Yamato, Tetsuo Minamino, yamato masaki, Takashi Matsuzaki, Ryo Araki, Shota Tsuchida, Keiji Okuda, Hai Ying Fu, Shoji Sanada, Hiroshi Asanuma, Yoshihiro Asano, Masanori Asakura, Hiroomi Torii, Kentaro Noi, Hirotsugu Ogi, Ryo Iwamoto, Eisuke Mekada, Seiji Takashima, Yasushi Sakata, Masafumi Kitakaze
  • Journal Title: Int. J. Gerontol. Volume: 11 Issue: 3 Pages: 191-196
  • DOI: 10.1016/j.ijge.2017.03.006
  • Peer Reviewed
• [Journal Article] Sequential Detection of Immunoglobulin G via Nonspecific Adsorbed Staphylococcal Protein A Using PDMS Quartz Crystal Microbalance Sensor (2017)
  • Author(s): Fumihito Kato, Hiroyuki Noguchi, Jun Kishinami, Chihaya Kimura, Taichi Kobayashi, Takumi Kobayashi, Keita Komori, Hirotsugu Ogi
  • Journal Title: Proceedings of The 38th Symposium on Ultrasonic Electronics Volume: 38
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic characterization of amyloid-fibril formation of Amyloid beta peptide using total-internal-reflection fluorescence microscopy coupled with quartz-crystal microbalance biosensor (2017)
  • Author(s): Kentaro Noi and Hirotsugi Ogi
  • Journal Title: Proceedings of The 38th Symposium on Ultrasonic Electronics Volume: 38
  • Peer Reviewed / Open Access
• [Journal Article] Viscoelasticity response during fibrillation of amyloid β peptides on quartz crystal microbalance biosensor (2016)
  • Author(s): Yen-Ting Lai, Hirotsugu Ogi, Arihiro Iwata and Masahiko Hirao
  • Journal Title: Proceedings of Symposium on Ultrasonic Electronics Volume: 37
  • NAID: 120007148394
  • Peer Reviewed / Open Access
• [Journal Article] Nucleus factory on cavitation bubble for amyloid β fibril (2016)
  • Author(s): K. Nakajima, H. Ogi, K. Adachi, K. Noi, M. Hirao, H. Yagi, and Y. Goto
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 22015-22015
  • DOI: 10.1038/srep22015
  • NAID: 120006557627
  • Peer Reviewed / Open Access
• [Journal Article] Viscoelasticity evolution in protein layers during binding reactions evaluated by a high-frequency wireless and electrodeless QCM biosensor without dissipation (2015)
  • Author(s): T. Shagawa, H. Torii, F. Kato, H. Ogi, and M. Hirao
  • Journal Title: Japanese Journal of Applied Physics Volume: 54 Issue: 6 Pages: 096601-096601
  • DOI: 10.7567/jjap.54.068001
  • Peer Reviewed
• [Journal Article] 無線・無電極水晶振動子バイオセンサーの原理と応用 (2015)
  • Author(s): 荻 博次
  • Journal Title: 化学とマイクロ・ナノシステム学会誌 Volume: 14 Pages: 24-29
  • NAID: 40020420553
• [Presentation] Development of TIRFM coupled with wireless-electrodeless QCM biosensor:Find and sound out fibrillation and fibril-dissociation phenomena (2017)
  • Author(s): Hirotsugu Ogi
  • Organizer: IPR Seminar / RIKEN Symposium: New Frontiers in Protein Misfolding and Aggregation
  • Place of Presentation: 大阪大学タンパク質研究所（大阪府吹田市）
  • Year and Date: 2017-01-27
  • Int'l Joint Research / Invited
• [Presentation] Ultrasonic cavitation and fibrillation phenomenon of protein (2017)
  • Author(s): Hirotsugu Ogi
  • Organizer: Australian National University (ANU) & IPR 2nd Joint Symposium 2017 “PROTEIN STRUCTURE AND FUNCTION”
  • Int'l Joint Research / Invited
• [Presentation] Ultra-High-Frequency Wireless MEMS QCM Biosensor for Direct Detection of Biomarkers in Serum (2017)
  • Author(s): Hirotsugu Ogi
  • Organizer: 2017 IEEE International Ultrasonics Symposium
  • Int'l Joint Research
• [Presentation] 超音波による蛋白質の凝集制御 (2016)
  • Author(s): 荻 博次
  • Organizer: 日本応用物理学会第77回秋季大会学術講演会
  • Place of Presentation: 新潟朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2016-09-13
  • Invited
• [Presentation] 核依存アミロイド線維の凝集過程及び線維融解過程のTIRFM－QCMによる直接観察 (2016)
  • Author(s): 山田晃大朗、西岡大介、中島吉太郎、荻博次、平尾雅彦、後藤祐児
  • Organizer: 第16回日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-06-07
• [Presentation] UHF帯MEMS無線水晶振動子バイオセンサによる夾雑物中のマーカー蛋白質の無標識検出 (2016)
  • Author(s): 岩田有弘、鳥居宏臣、荻博次、平尾 雅彦
  • Organizer: 第63回応用物理学会春季学術講演会
  • Place of Presentation: 東京工業大学 大岡山キャンパス、目黒区
  • Year and Date: 2016-03-19
• [Presentation] Ultrafast Propagation of Amyloid beta Fibril in Oligomer Cloud (2015)
  • Author(s): Hirotsugu Ogi
  • Organizer: PROTEIN STRUCTURE AND FUNCTION (Joint Symposium between IPR and RSC)
  • Place of Presentation: Australian National University、オーストラリア
  • Year and Date: 2015-11-14
  • Int'l Joint Research / Invited
• [Presentation] Study on aggregation reactions of amyloid β peptides induced by ultrasonic irradiation and stirring agitation (2015)
  • Author(s): 中島吉太郎、荻博次、平尾雅彦、後藤祐児
  • Organizer: 第36回超音波エレクトロニクスの基礎と応用に関するシンポジウム
  • Place of Presentation: つくば国際会議場、つくば市
  • Year and Date: 2015-11-05
• [Presentation] Detection of target protein via aptamer electrostatically immobilized on wireless-electrodeless QCM biosensor chip (2015)
  • Author(s): 鳥居宏臣、荻博次、平尾雅彦、山戸昌樹、松崎高志、南野哲男
  • Organizer: 第36回超音波エレクトロニクスの基礎と応用に関するシンポジウム
  • Place of Presentation: つくば国際会議場、つくば市
  • Year and Date: 2015-11-05
• [Presentation] 500 MHz帯無線MEMS水晶振動子バイオセンサの開発と夾雑物中の標的タンパク質の無標識検出 (2015)
  • Author(s): 岩田有弘、鳥居宏臣、荻博次、平尾雅彦
  • Organizer: 第76回応用物理学会秋季学術講演会
  • Place of Presentation: 名古屋国際会議場、名古屋市
  • Year and Date: 2015-09-13