| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
Recently it became possible to measure Raman spectra of a single live cell using a standard Raman microscope. We developed a bio-Raman microscope in order to monitor the dynamics of cellular differentiation, proliferation, and apoptosis. To do so, living cells were incubated on the microscope stage. Using the bio-Raman microscope, we defined cellular states in a non-destructive and non-labeling manner. The obtained information of cellular conditions is very important to control cellular destinies aiming tissue engineering. Advanced mathematical methods, developed in this project, were found effective to spectral analysis in a general manner.
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