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Criopreservation of Medaka Eggs with use of Liquid Meniscus for Protecting Membrane

Research Project

Project/Area Number 15H03933
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Thermal engineering
Research InstitutionKyushu Institute of Technology

Principal Investigator

TSURUTA TAKAHARU  九州工業大学, 大学院工学研究院, 教授 (30172068)

Co-Investigator(Kenkyū-buntansha) 谷川 洋文  九州工業大学, 大学院工学研究院, 助教 (80197524)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywords凍結保存 / 魚卵 / 液体メニスカス / 凍害防御剤 / メダカ卵 / 置換導入 / 脱水冷凍 / 孵化
Outline of Final Research Achievements

The cryopreservation of fish eggs is an important subject in the field of fishery and preservation of biological species. This study shows the effectiveness of using the liquid meniscus formed around the egg for protecting its morphology. Freezing and thawing experiments of medaka eggs were performed under different freezing conditions, and the hatching rate of the egg was examined. Before freezing, cryoprotective materials should be introduced inside the eggs in order to reduce the damages caused by ice formation. We have developed an effective method to introduce the aqueous solution of glycerin as the cryoprotectant with use of two fine-capillary tubes, which increased the supercooling and minimized the ice-damages. As a result, the preservation of egg tissue was confirmed at immediately after thawing. However, one hour after thawing, tissue change occurred and it was not possible to confirm hatching. Fast removal of glycerin after thawing is a future subject.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (5 results)

All 2017 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Possibility of cryopreservation of medaka eggs using liquid meniscus2016

    • Author(s)
      Takaharu TSURUTA, Hiroki SANO, Hirofumi TANIGAWA
    • Journal Title

      Journal of Thermal Science and Technology

      Volume: 11 Issue: 3 Pages: JTST0039-JTST0039

    • DOI

      10.1299/jtst.2016jtst0039

    • NAID

      130005252706

    • ISSN
      1880-5566
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] メダカ卵の卵内凍結に及ぼす凍害防御剤の影響2017

    • Author(s)
      大西寮,鶴田隆治,谷川洋文
    • Organizer
      2017年度日本冷凍空調学会年次大会講演論文集
    • Related Report
      2017 Annual Research Report
  • [Presentation] On a Possibility of Cryopreservation of Medaka Eggs with Use of Liquid-Meniscus2016

    • Author(s)
      Hiroki SANO, Hirofumi TANIGAWA and Takaharu TSURUTA
    • Organizer
      The First Pacific Rim Thermal Engineering Conference
    • Place of Presentation
      Waikoloa, Hawai, USA
    • Year and Date
      2016-03-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 界面前進凍結における溶質の有効分配係数に及ぼす凍結速度の影響2015

    • Author(s)
      松岡和生,谷川洋文,鶴田隆治
    • Organizer
      日本機械学会熱工学コンファレンス2015
    • Place of Presentation
      大阪大学(吹田市,大阪府)
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] メダカ卵の液体メニスカスを用いた冷凍保存法の検証2015

    • Author(s)
      佐野広樹,鶴田隆治
    • Organizer
      第52回日本伝熱シンポジウム
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2015-06-03
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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