| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|
|---|
| Outline of Final Research Achievements |
Membrane fusion, which is the last step of vesicular trafficking, is mediated by SNARE proteins. In this study, we have revealed that fusion of recycling vesicles containing transferrin-transferrin receptor is regulated by t-SNARE proteins, SNAP-23/SNAP-25 in conjunction with the Exocyst tethering complex.
By taking advantage of the visible immunoprecipitation (VIP) assay, we have revealed the architectures of various protein complex, including the Exocyst complex, involved in protein trafficking within cells.
|
