| Project/Area Number |
15H04406
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
奈良 武司 いわき明星大学, 薬学部, 教授 (40276473)
稲垣 祐司 筑波大学, 計算科学研究センター, 教授 (50387958)
谷藤 吾朗 独立行政法人国立科学博物館, 動物研究部, 研究員 (70438480)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | 機能進化 / スプライシング / 分割イントロン / ゲノム / Giardia / Kipferlia / スプライセオソーム |
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| Outline of Final Research Achievements |
A protozoan parasite, Giardia intestinalis, previously considered as a primitive eukaryote, possesses novel introns with a split form (split introns) which are spliced by trans-splicing. We evolutionarily analyzed the genome and transcriptome of Giardia and found a new split intron of this type and suggested the presence of other candidates. We also sequenced and surveyed the genome and transcriptome of Kipferlia, a close relative of Giardia, and found several candidates of split introns. On the other hand, in order to search for spliceosomal factors participating in the trans-splicing of split introns, we try to isolate and purify spliceosomes from Giardia and to compare the spliceosomal components with those of other eukaryotic model organisms.
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