High temperature resistance of the grain-filling depending on low grain growth rate and high assimilation in rice

• Project/Area Number: 15H04444
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop production science
• Research Institution: Shimane University
• Principal Investigator: Kobata Tohru 島根大学, 生物資源科学部, 名誉教授 (60186723)
• Co-Investigator(Kenkyū-buntansha): 氏家 和広 島根大学, 学術研究院農生命科学系, 准教授 (60465276) ; 三ツ井 敏明 新潟大学, 自然科学系, 教授 (70183960)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: イネ / 高温 / 登熟 / 品質 / 子実増加速度 / 同化 / 品種間差 / イネコアコレクション / 穂培養 / 収量と品質 / 子実成長速度 / 高温耐性 / 収量 / 酵素活性 / 子実成長 / 日本のイネコアコレクション / 籾の充填速度

Outline of Final Research Achievements

High temperature resistance in rice grain yield and quality is expected. Cultivars of a less increase in the grain filling under high temperature conditions was selected from rice of the world and Japan rice core collection. The less increase in the grain filling rate was expected to result a high temperature resistance of the grain filling because of relief in a lack of assimilate supply to the grain and keeping starch accumulation in grains. When panicles from these collection cultivars were solution cultured under 30 C, grain filling rate indicated diverse variability. The variability for selected typical cultivars was evidenced under diverse temperature and pot cultivated conditions. Metabolic processes for starch synthesis would relate with the cultivar difference in a response of the grain filling to high temperatures.

Academic Significance and Societal Importance of the Research Achievements

今後も日本のような中緯度地域の稲作地域において温暖化による高温化が進行すると予測される．このような条件下で今後も安定した食料生産を行うためには栽培的対応とともに品種的改良が不可欠である．本研究結果は高温に最も影響されやすい子実成長を維持するための特性としての子実の温度反応性に着目し，品種的多様性とその利用可能性について明らかにすることができた．今後、圃場におけるこれらの性質の再現性の確認，もう一つの体制となりうる同化の維持の特性についてさらに解明することで高温耐性品種の開発につながる可能性がある．

Research Products

• [Journal Article] How elevated CO2 affects our nutrition in rice, and how we can deal with it (2019)
  • Author(s): Ujiie Kazuhiro、Ishimaru Ken、Hirotsu Naoki、Nagasaka Seiji、Miyakoshi Yuichi、Ota Masako、Tokida Takeshi、Sakai Hidemitsu、Usui Yasuhiro、Ono Keisuke、Kobayashi Kazuhiko、Nakano Hiroshi、Yoshinaga Satoshi、Kashiwagi Takayuki、Magoshi Jun
  • Journal Title: PLOS ONE Volume: 14 Issue: 3 Pages: e0212840-e0212840
  • DOI: 10.1371/journal.pone.0212840
  • NAID: 120006871778
  • Peer Reviewed / Open Access
• [Journal Article] Responses of grain filling in spring wheat and temperate-zone rice to temperature: Similarities and differences (2018)
  • Author(s): Kobata Tohru、Palta Jairo A.、Tanaka Tomoyuki、Ohnishi Masao、Maeda Miki、Ko? M?jde、Barut?ular Celaleddin
  • Journal Title: Field Crops Research Volume: 215 Pages: 187-199
  • DOI: 10.1016/j.fcr.2017.10.017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Harvest index is a critical factor influencing the grain yield of diverse wheat species under rain-fed conditions in the Mediterranean zone of southeastern Turkey and northern Syria (2018)
  • Author(s): Kobata Tohru、Koch Mcjde、Barutcular Celaleddin、Tanno Ken-ichi、Inagaki Masanori
  • Journal Title: Plant Production Science Volume: 21 Issue: 2 Pages: 71-82
  • DOI: 10.1080/1343943x.2018.1445534
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Proteomics analysis reveals non-controlled activation of photosynthesis and protein synthesis in a rice npp1 mutant under high temperature and elevated CO2 conditions (2018)
  • Author(s): Takuya INOMATA, Marouane BASLAM, Takahiro MASUI, Tsutomu KOSHU, Takeshi TAKAMATSU, Kentaro KANEKO, Javier POZUETA-ROMERO, Toshiaki MITSUI
  • Journal Title: International Journal of Molecular Sciences Volume: 19 Issue: 9 Pages: 2655-2655
  • DOI: 10.3390/ijms19092655
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Novel molecular and cell biological insights into function of rice α-amylase (2018)
  • Author(s): Mitsui Toshiaki、Ochiai Akihito、Yamakawa Hiromoto、Kaneko Kentaro、Kitajima-Koga Aya、Baslam Marouane
  • Journal Title: Amylase Volume: 2 Issue: 1 Pages: 30-38
  • DOI: 10.1515/amylase-2018-0004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Reduction in Spikelet Number and Fertility Causes Yield Vulnerability in High-Yielding Rice (2017)
  • Author(s): Tohru Kobata, Hibiki Ishi and Hiroyuki Iwasaki
  • Journal Title: Agronomy Journal Volume: 109 Issue: 1 Pages: 175-184
  • DOI: 10.2134/agronj2016.05.0274
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A Quantitative Method for Analyzing Nitrogen Assimilation in Soybeans (2017)
  • Author(s): Jin Kakiuchi and Tohru Kobata
  • Journal Title: Crop Science Volume: 57 Issue: 2 Pages: 903-913
  • DOI: 10.2135/cropsci2016.05.0373
  • Peer Reviewed
• [Presentation] 穂培養下で登熟速度の異なる日本のイネコアコレクション品種における高温下登熟速度の違いの検証 (2019)
  • Author(s): 西岡 幹人・本塚 伊織・ 氏家 和広・小葉田 亨.
  • Organizer: 日本作物学会第244回講演会
• [Presentation] イネにおける澱粉代謝関連酵素の応用分子細胞生物学的研究、イネにおける澱粉代謝関連酵素の応用分子細胞生物学的研究 (2019)
  • Author(s): 三ツ井敏明
  • Organizer: 平成30年度 日本応用糖質科学会北海道支部 シンポジウム
• [Presentation] ケイ酸施用時期が水稲の収量・品質に及ぼす影響． (2019)
  • Author(s): 氏家和広・塩田京介
  • Organizer: 第247回日本作物学会講演会
• [Presentation] イネにおける澱粉代謝関連酵素の応用分子細胞生物学的研究 (2018)
  • Author(s): 三ツ井敏明
  • Organizer: 日本応用糖質科学会全員集会
• [Presentation] 高温登熟耐性としての穂培養によるイネ子実重増加速度温度反応の品種間差 (2018)
  • Author(s): 桐村 史悠・阿部 夢実・小葉田 亨
  • Organizer: 日本作物学会第244回講演会
• [Presentation] 水稲の収量・品質を改善する遺伝子 TGW6 の紹介． (2018)
  • Author(s): 氏家和広
  • Organizer: 日本作物学会中国支部会
• [Presentation] What Factors Reduce the Yield Potentiality in High Yielding Rice? (2017)
  • Author(s): Kobata Tohru
  • Organizer: Asian Crop Science Association
  • Int'l Joint Research
• [Presentation] 高温登熟抵抗性としての低い子実乾物増加速度をもつイネ品種の検索 (2016)
  • Author(s): 小葉田 亨・阿部 夢実・ 岩本 崇志
  • Organizer: 日本作物学会第242回講演会
  • Place of Presentation: 龍谷大学(滋賀県)
  • Year and Date: 2016-09-10
• [Presentation] Low grain growth rate can be an effective strategy for relief from the impact of high temperatures on the grain-filling in rice (Oryza sativa L)? (2016)
  • Author(s): Kobata Tohru
  • Organizer: 7th International Crop Science Cogress
  • Place of Presentation: 北京（中国)
  • Year and Date: 2016-08-14
  • Int'l Joint Research
• [Book] Root Biology Chapter9: Optimizing growth and tolerance of date palm (Phoenix dactylifera L.) to drought, salinity and vascular fusarium-induced wilt (Fusarium oxysporum) by application of Arbuscular Mycorrhizal Fungi (AMF). (2018)
  • Author(s): Meddich A., Ait El-Mokhtar M., Bourzik W., Mitsui T., Baslam M. And Hafidi M.
  • Total Pages: 20
  • Publisher: Springer
• [Book] 「米の外観品質・食味－最新研究と改善技術－」第15章 高温耐性イネの開発戦略－澱粉代謝関連酵素の細胞分子生物学の視点から－ (2018)
  • Author(s): 三ツ井敏明・金古堅太郎・白矢武士
  • Total Pages: 14
  • Publisher: 養賢堂
• [Funded Workshop] 7th International Crop Science Congress (2016)
  • Place of Presentation: 北京市(中国)
  • Year and Date: 2016-08-14