| Project/Area Number |
15H04479
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
平賀 良知 広島工業大学, 生命学部, 教授 (10238347)
山本 真司 広島大学, 理学研究科, 特任助教 (50607348)
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| Research Collaborator |
TAKAGI Ryukichi 広島大学, 大学院理学研究科, 助教 (90304394)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | Agrobacterium / rice transformation / p-coumarylalcohol / dehydrogenase / catabolism / mutant / plasmid removal / enzyme / alcohol dehydrogenase / 感染遺伝子発現調節 / イネ形質転換 / クマリルアルコール代謝 / 分解代謝遺伝子 / 代謝遺伝子 / クマリルアルコール代謝遺伝子 |
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| Outline of Final Research Achievements |
The Agrobacterium strain C58 has been used for rice transformation. The strain shows high ability to degrade p-coumarylalcohol (PCAL) that is secreted by rice cells and induces Agrobacterium vir genes. This study was conducted to enhance its transformation ability by revealing its genes for PCAL degradation. Removal of six putative dehydrogenase genes decreased PCAL dehydrogenase activity and degrading ability, and increased vir gene expression in response to co-cultivation with rice cali. The enzymes were found highly specific to PCAL. In addition to the multi gene family character, the data suggest physiological and ecological roles of the PCAL catabolism in the interaction between plant and Agrobacterium. In addition, this study invented a new and powerful technique to remove highly stable plasmid DNAs from strains.
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