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Ecology and diversity of Hymenoscyphus fraxineus, the causal agent of ash dieback in Europe in the areas near origin, and risk assessment as invasive plant pathogen

Research Project

Project/Area Number 15H04506
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Forest science
Research InstitutionUniversity of Tsukuba

Principal Investigator

YAMAOKA Yuichi  筑波大学, 生命環境系, 教授 (00220236)

Co-Investigator(Kenkyū-buntansha) 出川 洋介  筑波大学, 生命環境系, 准教授 (00311431)
細江 智夫  星薬科大学, 薬学部, 教授 (10287849)
石賀 康博  筑波大学, 生命環境系, 助教 (50730256)
岡根 泉  筑波大学, 生命環境系, 准教授 (60260171)
細矢 剛  独立行政法人国立科学博物館, 植物研究部, グループ長 (60392536)
Co-Investigator(Renkei-kenkyūsha) MASUYA Hayato  (独)森林総合研究所, 東北支所, チーム長 (70391183)
ABE Junichi  筑波大学, 生命環境系, 助教 (40292510)
Research Collaborator TAMAI Yutaka  北海道大学, (連合)農学研究科, 准教授 (50281796)
YAMAGUCHI Takehiro  (独)森林総合研究所, 北海道支所, チーム長 (00353897)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords菌類 / 植物病理学 / 樹病学 / 分類学 / 内生菌 / 生活環 / 侵略的外来種 / 病理学 / 林学
Outline of Final Research Achievements

Hymenoscyphus fraxineus causeing a lethal disease known as ‘ash dieback’ of Fraxinus excelsior in Europe is considered to be originated from East Asia. In Japan, this fungus produces apothecia on decomposing leaves of manshurian ash, F. mandshurica from middle July to early September. Results of detection of the fungus from periodically collected leaves of F. mandshurica by real-time PCR assays using species specific primers revealed that after infected the living leaves of by ascospores, H. fraxineus endophytically inhabited in them until defoliation. This fungus was also able to infect other Fraxinus species, including F. excelsior, F. pennsylvanica, and F. augustifolia, but no serious damages like ash dieback were observed in these ashes except for one population of F. pennsylvanica in Hokkaido. Some resistant reactions controlling movement of the fungus into stem tissues and/or environmental factor like overwinter condition might affect on symptom development.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (9 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Presentation (8 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Int'l Joint Research] Nor. Inst. Bioeconomy Research (NIBIO)(ノルウェー)

    • Related Report
      2016 Annual Research Report
  • [Presentation] 北海道における複数種のトネリコ属複葉内のHymenoscyphus fraxineusの挙動2018

    • Author(s)
      加藤早織, 岡根 泉, 玉井 裕, 山口岳広, 石賀康博, 山岡裕一
    • Organizer
      日本菌学会第62回大会(信州)
    • Related Report
      2017 Annual Research Report
  • [Presentation] An ash dieback pathogen, Hymenoscyphus fraxineus, endophytically inhabits leaves of manshurian ash in Japan2018

    • Author(s)
      Takahiro Inoue, Izumi Okane, Tsuyoshi Hosoya, Yosuke Degawa, Yasuhiro Ishiga, Yuichi Yamaoka
    • Organizer
      11th International Mycological Congress(IMC11)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hymenoscyphus fraxineusはヤチダモの生葉に内生する2017

    • Author(s)
      岡根 泉, 井上貴仁, 石賀康博, 出川洋介, 細矢 剛, 山岡裕一
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 国内ヤチダモ落枝葉から単離されたHymenoscyphus fraxineus のviridiol産生能2017

    • Author(s)
      若菜大悟, 武田 尚, 細江智夫, 山岡裕一, 岡根 泉, 出川洋介, 阿部淳一 , 細矢 剛, 升屋勇人
    • Organizer
      環境微生物系学会合同大会2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Inoculation experiments with Japanese isolates of Hymenoscyphus fraxineus2016

    • Author(s)
      Yamaoka, Y. and Okane, I.
    • Organizer
      Ash dieback seminar
    • Place of Presentation
      NIBIO, As, Norway
    • Year and Date
      2016-08-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ash dieback pathogen, Hymenoscyphus fraxineus endophytically existed in the leaves of Fraxinus mandshurica in Japan2016

    • Author(s)
      Okane, I. and Yamaoka, Y.
    • Organizer
      Ash dieback seminar
    • Place of Presentation
      NIBIO, As, Norway
    • Year and Date
      2016-08-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 日本産Hymenoscyphus fraxineusに対するヤチダモ及びセイヨウトネリコの反応2016

    • Author(s)
      井上貴仁・岡根泉・阿部淳一ピーター・石賀康博・細矢剛・山岡裕一
    • Organizer
      平成28年度日本植物病理学会大会
    • Place of Presentation
      岡山県岡山市 岡山コンベンションセンター
    • Year and Date
      2016-03-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Ash dieback pathogen, Hymenoscyphus fraxineus endophytically existed in the leaves of Fraxinus mandshurica in Japan.2015

    • Author(s)
      Inoue, T., Okane, I., Ishiga, Y., Deagawa, D., Hosoya, T. and Yamaoka, Y.
    • Organizer
      Asian Mycological Congress 2015
    • Place of Presentation
      Goa University, Goa, India
    • Year and Date
      2015-10-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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