Comparative genome analysis of Bursaphelenchus xylophilus in introduced and native areas.

• Project/Area Number: 15H04521
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Forest science
• Research Institution: Forest Research and Management Organization
• Principal Investigator: AKIBA MITSUTERU 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (50353553)
• Co-Investigator(Kenkyū-buntansha): 菊地 泰生 宮崎大学, 医学部, 准教授 (20353659) ; 神崎 菜摘 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (70435585)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000); Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: マツノザイセンチュウ / マツ材線虫病 / 比較ゲノム / 侵入病害 / ゲノム

Outline of Final Research Achievements

To understand the evolutional history of Bursaphelenchus xylophilus, the causal pathogen of the pine wilt disease, we performed population and comparative analyses of genomes of the nematodes using Japanese isolates and native North American isolates. Population analysis using microsatellite and SNPs suggested high genetic diversity among Japanese isolates and multiple introductions of the pathogen from North America to Japan.

Research Products

• [Journal Article] Loop-mediated isothermal amplification (LAMP) for detection of the red ring nematode, Bursaphelenchus cocophilus (2017)
  • Author(s): Ide Tatsuya、Kanzaki Natsumi、Parra Giraldo Pedro Pablo、Giblin-Davis Robin M.
  • Journal Title: Nematology Volume: 19 Issue: 5 Pages: 559-565
  • DOI: 10.1163/15685411-00003069
  • Peer Reviewed
• [Journal Article] Aphelenchoides eldaricus n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus eldarica in western Iran (2017)
  • Author(s): Esmaeili Mehrab、Heydari Ramin、Golhasan Behrouz、Kanzaki Natsumi
  • Journal Title: Nematology Volume: 19 Issue: 5 Pages: 605-616
  • DOI: 10.1163/15685411-00003074
  • Peer Reviewed
• [Journal Article] Distribution and community structure of soil nematodes in coastal Japanese pine forests were shaped by harsh environmental conditions (2017)
  • Author(s): Kitagami Yudai、Kanzaki Natsumi、Matsuda Yosuke
  • Journal Title: Applied Soil Ecology Volume: 119 Pages: 91-98
  • DOI: 10.1016/j.apsoil.2017.05.030
  • Peer Reviewed
• [Journal Article] Proposal of Bursaphelenchus wuae n. comb. and its two junior synonyms (2017)
  • Author(s): Gu Jianfeng、Kanzaki Natsumi、Tomalak Marek
  • Journal Title: Nematology Volume: 19 Issue: 7 Pages: 869-870
  • DOI: 10.1163/15685411-00003091
  • Peer Reviewed
• [Journal Article] マツノザイセンチュウとその近縁種の耐久型幼虫形態 (2017)
  • Author(s): 神崎菜摘、前原紀敏、秋庭満輝、田中克、井手竜也
  • Journal Title: 森林防疫 Volume: 66 Pages: 80-85
  • Peer Reviewed
• [Journal Article] Bursaphelenchus kesiyae n. sp (Nematoda: Aphelenchoididae), isolated from dead wood of Pinus kesiya Royle ex Gordon (Pinaceae) from Vietnam, with proposal of new subgroups in the B. fungivorus group (2016)
  • Author(s): Kanzaki, N., Aikawa, T., Maehara, N. & Thu, P.Q.
  • Journal Title: Nematology Volume: 18 Issue: 3 Pages: 133-146
  • DOI: 10.1163/15685411-00003051
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 日本におけるニセマツノザイセンチュウ２亜種の存在 (2017)
  • Author(s): 秋庭満輝、神崎菜摘、大澤正嗣
  • Organizer: 第25回日本線虫学会大会
• [Presentation] 沖縄島におけるマツノザイセンチュウの遺伝的構造 (2017)
  • Author(s): 秋庭満輝、伊藤俊輔 、佐橋憲生
  • Organizer: 第128回日本森林学会大会
• [Presentation] 沖縄島におけるマツノザイセンチュウの遺伝的多様性 (2016)
  • Author(s): 秋庭満輝、伊藤俊輔
  • Organizer: 第24回日本線虫学会大会
• [Presentation] ヒゲナガカミキリおよびマツノマダラカミキリからのBursaphelenchus firmaeおよびDiplogasteroides andrassyiの分離 (2015)
  • Author(s): 秋庭満輝、神崎菜摘
  • Organizer: 日本線虫学会第23回大会
  • Place of Presentation: 中部大学
  • Year and Date: 2015-09-03