Development of high sensitive method for detection of cleanliness on meat surface by the excitation-emission matrix

Research Project

Project/Area Number	15H04576
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Agricultural environmental engineering/Agricultural information engineering
Research Institution	The University of Tokyo
Principal Investigator	Oshita Seiichi 東京大学, 大学院農学生命科学研究科(農学部), 特任教授 (00115693)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000) Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords	食肉 / 一般生菌数 / ATP / 励起蛍光マトリクス / 清浄度評価 / NADPH / 非破壊計測
Outline of Final Research Achievements	In order to estimate the plate count on pork meat surface, the excitation-emission matrix (EEM) obtained from spectroscopy data was measured. At the same time, both ATP content with bioluminescence method and plate count with swabbing method were also measured. The two-dimensional Savitzky-Golay second-order differentiation was applied to EEM data, then PLSR model was developed for estimating plate count by using EEM data. The PLSR model showed high correlation for the estimation of plate count with correlation coefficient of 0.94 and RMSEP=log10(0.60, mol cm-2) for all the data from 0 h to 72h. Focusing on the initial preservation stage from 0 h to 48 h, the PLSR model gave the correlation coefficient of 0.89 and RMSEP=log10(0.62, mol cm-2).