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Study on mechanism for freeze trelance of a leech Ozobranchus jantseanus and its application

Research Project

Project/Area Number 15H04577
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

SUZUKI Toru  東京海洋大学, 学術研究院, 教授 (50206504)

Co-Investigator(Kenkyū-buntansha) 川井 清司  広島大学, 生物圏科学研究科, 准教授 (00454140)
萩原 知明  東京海洋大学, 学術研究院, 教授 (20293095)
黄川田 隆洋  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (60414900)
鈴木 大  東海大学, 生物学部, 講師 (90647489)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywordsヌマエラビル / 耐凍性 / カルノシン / 凍結耐性 / 冷凍 / 飼育 / 遺伝子解析
Outline of Final Research Achievements

A big result in this research was that breeding method of a leech Ozobranchus jantseanus was established in this study. From a past and this studies, it was known that the leech extract (including carnosine) not having an effect to control ice crystal growth. On the other hand, a lot of damaged cells in the organ frozen leech was found in the leech showing high ability to restore. These results suggest that the leech dose not restrain damage due to ice crystalization in freezing, but having a high reconstruction characteristics in thawing. Indeed, there is a possibility that carnosine having antioxidant ability found by metabolic loam analysis participates in it. The applied effect such as carnosine improving the lyophilization tolerance of the lactic acid bacterium may be one side to support it.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (6 results)

All 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] 保護物質による凍結乾燥酵素および乳酸菌の安定化2017

    • Author(s)
      川井清司
    • Journal Title

      冷凍

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] 凍結耐性を示すヌマエラビルの細胞組織変化及び飼育法に関する研究2017

    • Author(s)
      関口 由起, 原川 明宏, 鈴木 大, 鈴木 徹
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effect of protectants on the storage stability of freeze-dried lactic acid bacteria2017

    • Author(s)
      S. Mikajiri, K. Kawai, T. Da, Y. Hagura, D. Suzuki, T. Hagiwara, T. Kikawada, T. Suzuki
    • Organizer
      Food Innova 2017
    • Place of Presentation
      Cesena, Italy
    • Year and Date
      2017-01-31
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高い凍結耐性を持つヌマエラビル(Ozobranchus jantseanus)の細胞組織変化2017

    • Author(s)
      鈴木 徹, 関口 由起, 原川 昭宏, 鈴木 大
    • Organizer
      第62回低温生物工学会, 北海道大学(札幌)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 保護物質が凍結乾燥乳酸菌の生菌数に及ぼす影響2017

    • Author(s)
      川井 清司, 三ヶ尻 脩人, 滕 達, 羽倉 義雄, 鈴木 大, 萩原 知明, 黄川田 隆洋, 鈴木 徹
    • Organizer
      第62回低温生物工学会, 北海道大学(札幌)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Stabilization of Freeze-dried Functional Materials and Lactobacillus by Vitrification2016

    • Author(s)
      K. Kawai, Y. Hagura
    • Organizer
      International Food Machinery and Technology Exhibition
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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