| Project/Area Number |
15H04586
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Animal production science
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
Aso Hisashi 東北大学, 農学研究科, 教授 (50241625)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
北澤 春樹 東北大学, 農学研究科, 准教授 (10204885)
奥 夏美 (岡田夏美 / 岡田 夏美) 東北大学, 農学研究科, 技術一般職員 (10621584)
野地 智法 東北大学, 農学研究科, 准教授 (10708001)
渡邊 康一 東北大学, 農学研究科, 助教 (80261494)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
|---|
| Keywords | プリオン / M細胞 / アルドラーゼA / ウシ腸管上皮細胞 / マウス腸管上皮細胞 / 侵入 / トランスサイトーシス / 抗体阻害 / 牛腸管上皮細胞株 / マウス腸管上皮細胞株 / 受容体 / 複合体 |
|---|
| Outline of Final Research Achievements |
BSE is a fatal neurodegenerative disorder in cattle and is linked to variant Creutzfeldt-Jakob disease in humans. We show that M cells can incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. The results of 2-DE show a specific protein associated with the PrP-coated particles. This protein was identified as aldolase A using LC-MS/MS analysis. The binding of rbPrP to aldolase A was inhibited by pre-treatment of anti-aldolase A antibody. We established the aldolase-knockout cell line (MIE ALDOA -/- cells) by TALEN method. After the differentiation for M cell, MIE ALDOA -/- cells lacked the ability of transporting PrP coated magnetic particles into the basal media through the intracellular vesicles.
These results suggest that bovine M cells synthesize aldolase A, and that aldolase A-positive M cells may play a key role in the invasion of BSE into the body.
|
